TA320469

antibody from OriGene

Targeting: TBX21 T-bet, TBLYM

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA320469

Provider

OriGene

Product name

Mouse Anti-T-bet Purified (25 ug)

Antibody type

Monoclonal

Description

Mouse Anti-T-bet Purified (25 ug)

Host

Mouse

Conjugate

Unconjugated

Epitope

TBX21

Antibody clone number

eBio39D (39D, 3-9D)

Vial size

25 µg

Concentration

0.5-1mg/ml (lot dependent)

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

CD4+ T cells were sorted from mouse splenocytes, then activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-stimulation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-stimulated (right) cells were probed with Anti-T-bet Purified at 2 ug/ml, and revealed with Anti-Mouse IgG HRP.

Validation comment

WB