Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- IC53851G-025 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal T-bet/TBX21 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human T-bet/TBX21 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of T-bet/TBX21 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD8 alpha APC-conjugated Monoclonal Antibody (Catalog # FAB1509A) and either (A) Mouse Anti-Human T-bet/TBX21 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC53851G) or (B) Mouse IgG1 Alexa Fluor 488 Isotype Control (Catalog # IC002G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of T-bet/TBX21 in CD4 + Human PBMCs Stimulated to Induce Th1 Cells by Flow Cytometry. CD4+ Human peripheral blood mononuclear cells (PBMCs) treated with 20 ng/mL Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA) and 10 ng/mL Recombinant Human IL-12 (Catalog # 219-IL) for 5 days to induce Th1 cell development for were stained with Mouse Anti-Human IFN-gamma PE-conjugated Monoclonal Antibody (Catalog # IC285P) and either (A) Mouse Anti-Human T-bet/TBX21 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC53851G) or (B) Mouse IgG1 Alexa Fluor 488 Isotype Control (Catalog # IC002G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.