IC53851G-025

antibody from R&D Systems

Targeting: TBX21 T-bet, TBLYM

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC53851G-025

Provider

R&D Systems

Product name

Human T-bet/TBX21 Alexa Fluor® 488-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from cell culture supernatant. Detects human T-bet/TBX21 in direct ELISAs.

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Antigen sequence

Q9UL17


Isotype

IgG

Antibody clone number

525831

Vial size

25 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of T-bet/TBX21 in CD4 ⁺ Human PBMCs Stimulated to Induce Th1 Cells by Flow Cytometry. CD4⁺ Human peripheral blood mononuclear cells (PBMCs) treated with 20 ng/mL Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA) and 10 ng/mL Recombinant Human IL-12 (Catalog # 219-IL) for 5 days to induce Th1 cell development for were stained with Mouse Anti-Human IFN-gamma PE-conjugated Monoclonal Antibody (Catalog # IC285P) and either (A) Mouse Anti-Human T-bet/TBX21 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC53851G) or (B) Mouse IgG₁ Alexa Fluor 488 Isotype Control (Catalog # IC002G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of T-bet/TBX21 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD8 alpha APC-conjugated Monoclonal Antibody (Catalog # FAB1509A) and either (A) Mouse Anti-Human T-bet/TBX21 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC53851G) or (B) Mouse IgG₁ Alexa Fluor 488 Isotype Control (Catalog # IC002G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.