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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C106601 - Provider product page
- Provider
- LSBio
- Product name
- TBX21 / T-bet Antibody (clone 4B10, 4-B10) LS-C106601
- Antibody type
- Monoclonal
- Description
- Affinity purified
- Reactivity
- Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4B10, 4-B10
- Storage
- Store at 4°C, avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Main image
- Experimental details
- CD4+ T cells were sorted from mouse spleen, activated with anti-mouse CD3 and anti-mouse CD28, followed by culture in Th1-polarizing conditions, and re-activation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-activated (right) cells were probed with purified antibody at 2 ug/ml, and revealed with HRP anti-mouse IgG.
