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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 644831 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#644831, RRID:AB_2572010
- Product name
- Direct-Blot™ HRP anti-T-bet
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Mouse
- Antibody clone number
- 4B10
- Vial size
- 100 µl (20 Western blots)
- Storage
- -20°C
- Handling
- Blue Ice
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Protein extracted from PBMC treated with 5µg/mL CD3 and 2µg/mL CD28 was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ anti-HRP T-bet (clone 4B10). Proteins were visualized using chemiluminescence detection.
