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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [61]
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- Validations
- Flow cytometry [1]
- Other assay [15]
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- Product number
- 25-5825-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- T-bet Monoclonal Antibody (eBio4B10 (4B10)), PE-Cyanine7, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells. Applications Reported: This eBio4B10 (4B10, 4-B10) antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eBio4B10 (4B10, 4-B10) antibody has been tested by intracellular stainign and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocol section; (refer to Protocol B: One step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBio4B10 (4B10)
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Interleukin-1β secretion induced by mucosa-associated gut commensal bacteria promotes intestinal barrier repair.
Culture, expansion, and flow-cytometry-based functional analysis of pteropid bat MR1-restricted unconventional T cells.
Francisella tularensis induces Th1 like MAIT cells conferring protection against systemic and local infection.
DNA Methylation Patterns in CD4+ T Cells of Naïve and Influenza A Virus-Infected Mice Developmentally Exposed to an Aryl Hydrocarbon Receptor Ligand.
ADAM12 is a costimulatory molecule that determines Th1 cell fate and mediates tissue inflammation.
MiR-1165-3p Suppresses Th2 Differentiation via Targeting IL-13 and PPM1A in a Mouse Model of Allergic Airway Inflammation.
Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver.
Continued Bcl6 Expression Prevents the Transdifferentiation of Established Tfh Cells into Th1 Cells during Acute Viral Infection.
Long-Term Programming of CD8 T Cell Immunity by Perinatal Exposure to Glucocorticoids.
Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease.
Salt Sensing by Serum/Glucocorticoid-Regulated Kinase 1 Promotes Th17-like Inflammatory Adaptation of Foxp3(+) Regulatory T Cells.
Niche-Selective Inhibition of Pathogenic Th17 Cells by Targeting Metabolic Redundancy.
Single-cell RNA sequencing identifies shared differentiation paths of mouse thymic innate T cells.
Blockade of the AHR restricts a Treg-macrophage suppressive axis induced by L-Kynurenine.
PD-1 Blockade Reinvigorates Bone Marrow CD8(+) T Cells from Patients with Multiple Myeloma in the Presence of TGFβ Inhibitors.
Development of Hematopoietic Stem Cell-Engineered Invariant Natural Killer T Cell Therapy for Cancer.
A chemical biology toolbox to study protein methyltransferases and epigenetic signaling.
A functional subset of CD8(+) T cells during chronic exhaustion is defined by SIRPα expression.
Qa-1-Restricted CD8(+) T Cells Can Compensate for the Absence of Conventional T Cells during Viral Infection.
An Id2(RFP)-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.
Limited Foxp3(+) Regulatory T Cells Response During Acute Trypanosoma cruzi Infection Is Required to Allow the Emergence of Robust Parasite-Specific CD8(+) T Cell Immunity.
Transcription factors early growth response gene (Egr) 2 and 3 control inflammatory responses of tolerant T cells.
Non-conventional Inhibitory CD4(+)Foxp3(-)PD-1(hi) T Cells as a Biomarker of Immune Checkpoint Blockade Activity.
Critical Role for the Microbiota in CX(3)CR1(+) Intestinal Mononuclear Phagocyte Regulation of Intestinal T Cell Responses.
Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.
Regulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis.
Conventional NK cells and ILC1 are partially ablated in the livers of Ncr1 (iCre)Tbx21 (fl/fl) mice.
Antibiotic-induced perturbations in microbial diversity during post-natal development alters amyloid pathology in an aged APP(SWE)/PS1(ΔE9) murine model of Alzheimer's disease.
Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation.
Resistance to TGFβ suppression and improved anti-tumor responses in CD8(+) T cells lacking PTPN22.
MR1-restricted mucosal-associated invariant T (MAIT) cells respond to mycobacterial vaccination and infection in nonhuman primates.
mTORC2 regulates multiple aspects of NKT-cell development and function.
The microvascular niche instructs T cells in large vessel vasculitis via the VEGF-Jagged1-Notch pathway.
Mucosal-associated invariant T-cell activation and accumulation after in vivo infection depends on microbial riboflavin synthesis and co-stimulatory signals.
Intestinal helminth infection induces highly functional resident memory CD4(+) T cells in mice.
Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.
A Mouse Model of West Nile Virus Infection.
Sex impacts Th1 cells, Tregs, and DCs in both intestinal and systemic immunity in a mouse strain and location-dependent manner.
Clonal expansion of CD4(+) cytotoxic T lymphocytes in patients with IgG4-related disease.
CD4(+) T-cell survival in the GI tract requires dectin-1 during fungal infection.
B-cell anergy induces a Th17 shift in a novel B lymphocyte transgenic NOD mouse model, the 116C-NOD mouse.
Treatment with cyclophosphamide supported by various dendritic cell-based vaccines induces diversification in CD4⁺ T cell response against MC38 colon carcinoma.
Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.
Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation.
Oxygen Sensing by T Cells Establishes an Immunologically Tolerant Metastatic Niche.
Protein Translation Activity: A New Measure of Host Immune Cell Activation.
Intranasal Administration of Lentiviral miR-135a Regulates Mast Cell and Allergen-Induced Inflammation by Targeting GATA-3.
Virulence-Dependent Alterations in the Kinetics of Immune Cells during Pulmonary Infection by Mycobacterium tuberculosis.
Triclosan Induces Thymic Stromal Lymphopoietin in Skin Promoting Th2 Allergic Responses.
Reciprocal regulation of lymphoid tissue development in the large intestine by IL-25 and IL-23.
Th17-type cytokines, IL-6 and TNF-α synergistically activate STAT3 and NF-kB to promote colorectal cancer cell growth.
The transduction pattern of IL-12-encoding lentiviral vectors shapes the immunological outcome.
CD4CD8αα lymphocytes, a novel human regulatory T cell subset induced by colonic bacteria and deficient in patients with inflammatory bowel disease.
MyD88 signaling in CD4 T cells promotes IFN-γ production and hematopoietic progenitor cell expansion in response to intracellular bacterial infection.
NK cell maturation and function in C57BL/6 mice are altered by caloric restriction.
Th17 effector cells support B cell responses outside of germinal centres.
Increase in Th17 and T-reg lymphocytes and decrease of IL22 correlate with the recovery phase of acute EAE in rat.
CD134 plus CD137 dual costimulation induces Eomesodermin in CD4 T cells to program cytotoxic Th1 differentiation.
Cytokine requirements for the differentiation and expansion of IL-17A- and IL-22-producing human Vgamma2Vdelta2 T cells.
Differential expression of T-bet, a T-box transcription factor required for Th1 T-cell development, in peripheral T-cell lymphomas.
A novel transcription factor, T-bet, directs Th1 lineage commitment.
Wu WH, Kim M, Chang LC, Assie A, Saldana-Morales FB, Zegarra-Ruiz DF, Norwood K, Samuel BS, Diehl GE
Gut microbes 2022 Jan-Dec;14(1):2014772
Gut microbes 2022 Jan-Dec;14(1):2014772
Culture, expansion, and flow-cytometry-based functional analysis of pteropid bat MR1-restricted unconventional T cells.
Sia WR, Hey YY, Foo R, Wang LF, Leeansyah E
STAR protocols 2021 Jun 18;2(2):100487
STAR protocols 2021 Jun 18;2(2):100487
Francisella tularensis induces Th1 like MAIT cells conferring protection against systemic and local infection.
Zhao Z, Wang H, Shi M, Zhu T, Pediongco T, Lim XY, Meehan BS, Nelson AG, Fairlie DP, Mak JYW, Eckle SBG, de Lima Moreira M, Tumpach C, Bramhall M, Williams CG, Lee HJ, Haque A, Evrard M, Rossjohn J, McCluskey J, Corbett AJ, Chen Z
Nature communications 2021 Jul 16;12(1):4355
Nature communications 2021 Jul 16;12(1):4355
DNA Methylation Patterns in CD4+ T Cells of Naïve and Influenza A Virus-Infected Mice Developmentally Exposed to an Aryl Hydrocarbon Receptor Ligand.
Burke CG, Myers JR, Post CM, Boulé LA, Lawrence BP
Environmental health perspectives 2021 Jan;129(1):17007
Environmental health perspectives 2021 Jan;129(1):17007
ADAM12 is a costimulatory molecule that determines Th1 cell fate and mediates tissue inflammation.
Liu Y, Bockermann R, Hadi M, Safari I, Carrion B, Kveiborg M, Issazadeh-Navikas S
Cellular & molecular immunology 2021 Aug;18(8):1904-1919
Cellular & molecular immunology 2021 Aug;18(8):1904-1919
MiR-1165-3p Suppresses Th2 Differentiation via Targeting IL-13 and PPM1A in a Mouse Model of Allergic Airway Inflammation.
Wang Z, Ji N, Chen Z, Sun Z, Wu C, Yu W, Hu F, Huang M, Zhang M
Allergy, asthma & immunology research 2020 Sep;12(5):859-876
Allergy, asthma & immunology research 2020 Sep;12(5):859-876
Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver.
Remmerie A, Martens L, Thoné T, Castoldi A, Seurinck R, Pavie B, Roels J, Vanneste B, De Prijck S, Vanhockerhout M, Binte Abdul Latib M, Devisscher L, Hoorens A, Bonnardel J, Vandamme N, Kremer A, Borghgraef P, Van Vlierberghe H, Lippens S, Pearce E, Saeys Y, Scott CL
Immunity 2020 Sep 15;53(3):641-657.e14
Immunity 2020 Sep 15;53(3):641-657.e14
Continued Bcl6 Expression Prevents the Transdifferentiation of Established Tfh Cells into Th1 Cells during Acute Viral Infection.
Alterauge D, Bagnoli JW, Dahlström F, Bradford BM, Mabbott NA, Buch T, Enard W, Baumjohann D
Cell reports 2020 Oct 6;33(1):108232
Cell reports 2020 Oct 6;33(1):108232
Long-Term Programming of CD8 T Cell Immunity by Perinatal Exposure to Glucocorticoids.
Hong JY, Lim J, Carvalho F, Cho JY, Vaidyanathan B, Yu S, Annicelli C, Ip WKE, Medzhitov R
Cell 2020 Mar 5;180(5):847-861.e15
Cell 2020 Mar 5;180(5):847-861.e15
Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease.
Lee JY, Hall JA, Kroehling L, Wu L, Najar T, Nguyen HH, Lin WY, Yeung ST, Silva HM, Li D, Hine A, Loke P, Hudesman D, Martin JC, Kenigsberg E, Merad M, Khanna KM, Littman DR
Cell 2020 Jan 9;180(1):79-91.e16
Cell 2020 Jan 9;180(1):79-91.e16
Salt Sensing by Serum/Glucocorticoid-Regulated Kinase 1 Promotes Th17-like Inflammatory Adaptation of Foxp3(+) Regulatory T Cells.
Yang YH, Istomine R, Alvarez F, Al-Aubodah TA, Shi XQ, Takano T, Thornton AM, Shevach EM, Zhang J, Piccirillo CA
Cell reports 2020 Feb 4;30(5):1515-1529.e4
Cell reports 2020 Feb 4;30(5):1515-1529.e4
Niche-Selective Inhibition of Pathogenic Th17 Cells by Targeting Metabolic Redundancy.
Wu L, Hollinshead KER, Hao Y, Au C, Kroehling L, Ng C, Lin WY, Li D, Silva HM, Shin J, Lafaille JJ, Possemato R, Pacold ME, Papagiannakopoulos T, Kimmelman AC, Satija R, Littman DR
Cell 2020 Aug 6;182(3):641-654.e20
Cell 2020 Aug 6;182(3):641-654.e20
Single-cell RNA sequencing identifies shared differentiation paths of mouse thymic innate T cells.
Lee M, Lee E, Han SK, Choi YH, Kwon DI, Choi H, Lee K, Park ES, Rha MS, Joo DJ, Shin EC, Kim S, Kim JK, Lee YJ
Nature communications 2020 Aug 31;11(1):4367
Nature communications 2020 Aug 31;11(1):4367
Blockade of the AHR restricts a Treg-macrophage suppressive axis induced by L-Kynurenine.
Campesato LF, Budhu S, Tchaicha J, Weng CH, Gigoux M, Cohen IJ, Redmond D, Mangarin L, Pourpe S, Liu C, Zappasodi R, Zamarin D, Cavanaugh J, Castro AC, Manfredi MG, McGovern K, Merghoub T, Wolchok JD
Nature communications 2020 Aug 11;11(1):4011
Nature communications 2020 Aug 11;11(1):4011
PD-1 Blockade Reinvigorates Bone Marrow CD8(+) T Cells from Patients with Multiple Myeloma in the Presence of TGFβ Inhibitors.
Kwon M, Kim CG, Lee H, Cho H, Kim Y, Lee EC, Choi SJ, Park J, Seo IH, Bogen B, Song IC, Jo DY, Kim JS, Park SH, Choi I, Choi YS, Shin EC
Clinical cancer research : an official journal of the American Association for Cancer Research 2020 Apr 1;26(7):1644-1655
Clinical cancer research : an official journal of the American Association for Cancer Research 2020 Apr 1;26(7):1644-1655
Development of Hematopoietic Stem Cell-Engineered Invariant Natural Killer T Cell Therapy for Cancer.
Zhu Y, Smith DJ, Zhou Y, Li YR, Yu J, Lee D, Wang YC, Di Biase S, Wang X, Hardoy C, Ku J, Tsao T, Lin LJ, Pham AT, Moon H, McLaughlin J, Cheng D, Hollis RP, Campo-Fernandez B, Urbinati F, Wei L, Pang L, Rezek V, Berent-Maoz B, Macabali MH, Gjertson D, Wang X, Galic Z, Kitchen SG, An DS, Hu-Lieskovan S, Kaplan-Lefko PJ, De Oliveira SN, Seet CS, Larson SM, Forman SJ, Heath JR, Zack JA, Crooks GM, Radu CG, Ribas A, Kohn DB, Witte ON, Yang L
Cell stem cell 2019 Oct 3;25(4):542-557.e9
Cell stem cell 2019 Oct 3;25(4):542-557.e9
A chemical biology toolbox to study protein methyltransferases and epigenetic signaling.
Scheer S, Ackloo S, Medina TS, Schapira M, Li F, Ward JA, Lewis AM, Northrop JP, Richardson PL, Kaniskan HÜ, Shen Y, Liu J, Smil D, McLeod D, Zepeda-Velazquez CA, Luo M, Jin J, Barsyte-Lovejoy D, Huber KVM, De Carvalho DD, Vedadi M, Zaph C, Brown PJ, Arrowsmith CH
Nature communications 2019 Jan 3;10(1):19
Nature communications 2019 Jan 3;10(1):19
A functional subset of CD8(+) T cells during chronic exhaustion is defined by SIRPα expression.
Myers LM, Tal MC, Torrez Dulgeroff LB, Carmody AB, Messer RJ, Gulati G, Yiu YY, Staron MM, Angel CL, Sinha R, Markovic M, Pham EA, Fram B, Ahmed A, Newman AM, Glenn JS, Davis MM, Kaech SM, Weissman IL, Hasenkrug KJ
Nature communications 2019 Feb 15;10(1):794
Nature communications 2019 Feb 15;10(1):794
Qa-1-Restricted CD8(+) T Cells Can Compensate for the Absence of Conventional T Cells during Viral Infection.
Anderson CK, Reilly EC, Lee AY, Brossay L
Cell reports 2019 Apr 9;27(2):537-548.e5
Cell reports 2019 Apr 9;27(2):537-548.e5
An Id2(RFP)-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.
Xu W, Cherrier DE, Chea S, Vosshenrich C, Serafini N, Petit M, Liu P, Golub R, Di Santo JP
Immunity 2019 Apr 16;50(4):1054-1068.e3
Immunity 2019 Apr 16;50(4):1054-1068.e3
Limited Foxp3(+) Regulatory T Cells Response During Acute Trypanosoma cruzi Infection Is Required to Allow the Emergence of Robust Parasite-Specific CD8(+) T Cell Immunity.
Araujo Furlan CL, Tosello Boari J, Rodriguez C, Canale FP, Fiocca Vernengo F, Boccardo S, Beccaria CG, Adoue V, Joffre O, Gruppi A, Montes CL, Acosta Rodriguez EV
Frontiers in immunology 2018;9:2555
Frontiers in immunology 2018;9:2555
Transcription factors early growth response gene (Egr) 2 and 3 control inflammatory responses of tolerant T cells.
Omodho B, Miao T, Symonds ALJ, Singh R, Li S, Wang P
Immunity, inflammation and disease 2018 Jun;6(2):221-233
Immunity, inflammation and disease 2018 Jun;6(2):221-233
Non-conventional Inhibitory CD4(+)Foxp3(-)PD-1(hi) T Cells as a Biomarker of Immune Checkpoint Blockade Activity.
Zappasodi R, Budhu S, Hellmann MD, Postow MA, Senbabaoglu Y, Manne S, Gasmi B, Liu C, Zhong H, Li Y, Huang AC, Hirschhorn-Cymerman D, Panageas KS, Wherry EJ, Merghoub T, Wolchok JD
Cancer cell 2018 Jun 11;33(6):1017-1032.e7
Cancer cell 2018 Jun 11;33(6):1017-1032.e7
Critical Role for the Microbiota in CX(3)CR1(+) Intestinal Mononuclear Phagocyte Regulation of Intestinal T Cell Responses.
Kim M, Galan C, Hill AA, Wu WJ, Fehlner-Peach H, Song HW, Schady D, Bettini ML, Simpson KW, Longman RS, Littman DR, Diehl GE
Immunity 2018 Jul 17;49(1):151-163.e5
Immunity 2018 Jul 17;49(1):151-163.e5
Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.
Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, Aid M, Tsui C, Maldonado P, Nair U, Ghneim K, Fallon PG, Sekaly RP, Barouch DH, Shalek AK, Bruckbauer A, Strid J, Batista FD
Cell 2018 Jan 25;172(3):517-533.e20
Cell 2018 Jan 25;172(3):517-533.e20
Regulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis.
Kim DH, Park HJ, Lim S, Koo JH, Lee HG, Choi JO, Oh JH, Ha SJ, Kang MJ, Lee CM, Lee CG, Elias JA, Choi JM
Nature communications 2018 Feb 5;9(1):503
Nature communications 2018 Feb 5;9(1):503
Conventional NK cells and ILC1 are partially ablated in the livers of Ncr1 (iCre)Tbx21 (fl/fl) mice.
Cuff AO, Male V
Wellcome open research 2017;2:39
Wellcome open research 2017;2:39
Antibiotic-induced perturbations in microbial diversity during post-natal development alters amyloid pathology in an aged APP(SWE)/PS1(ΔE9) murine model of Alzheimer's disease.
Minter MR, Hinterleitner R, Meisel M, Zhang C, Leone V, Zhang X, Oyler-Castrillo P, Zhang X, Musch MW, Shen X, Jabri B, Chang EB, Tanzi RE, Sisodia SS
Scientific reports 2017 Sep 5;7(1):10411
Scientific reports 2017 Sep 5;7(1):10411
Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation.
Wang S, Xia P, Chen Y, Qu Y, Xiong Z, Ye B, Du Y, Tian Y, Yin Z, Xu Z, Fan Z
Cell 2017 Sep 21;171(1):201-216.e18
Cell 2017 Sep 21;171(1):201-216.e18
Resistance to TGFβ suppression and improved anti-tumor responses in CD8(+) T cells lacking PTPN22.
Brownlie RJ, Garcia C, Ravasz M, Zehn D, Salmond RJ, Zamoyska R
Nature communications 2017 Nov 7;8(1):1343
Nature communications 2017 Nov 7;8(1):1343
MR1-restricted mucosal-associated invariant T (MAIT) cells respond to mycobacterial vaccination and infection in nonhuman primates.
Greene JM, Dash P, Roy S, McMurtrey C, Awad W, Reed JS, Hammond KB, Abdulhaqq S, Wu HL, Burwitz BJ, Roth BF, Morrow DW, Ford JC, Xu G, Bae JY, Crank H, Legasse AW, Dang TH, Greenaway HY, Kurniawan M, Gold MC, Harriff MJ, Lewinsohn DA, Park BS, Axthelm MK, Stanton JJ, Hansen SG, Picker LJ, Venturi V, Hildebrand W, Thomas PG, Lewinsohn DM, Adams EJ, Sacha JB
Mucosal immunology 2017 May;10(3):802-813
Mucosal immunology 2017 May;10(3):802-813
mTORC2 regulates multiple aspects of NKT-cell development and function.
Sklarz T, Guan P, Gohil M, Cotton RM, Ge MQ, Haczku A, Das R, Jordan MS
European journal of immunology 2017 Mar;47(3):516-526
European journal of immunology 2017 Mar;47(3):516-526
The microvascular niche instructs T cells in large vessel vasculitis via the VEGF-Jagged1-Notch pathway.
Wen Z, Shen Y, Berry G, Shahram F, Li Y, Watanabe R, Liao YJ, Goronzy JJ, Weyand CM
Science translational medicine 2017 Jul 19;9(399)
Science translational medicine 2017 Jul 19;9(399)
Mucosal-associated invariant T-cell activation and accumulation after in vivo infection depends on microbial riboflavin synthesis and co-stimulatory signals.
Chen Z, Wang H, D'Souza C, Sun S, Kostenko L, Eckle SB, Meehan BS, Jackson DC, Strugnell RA, Cao H, Wang N, Fairlie DP, Liu L, Godfrey DI, Rossjohn J, McCluskey J, Corbett AJ
Mucosal immunology 2017 Jan;10(1):58-68
Mucosal immunology 2017 Jan;10(1):58-68
Intestinal helminth infection induces highly functional resident memory CD4(+) T cells in mice.
Steinfelder S, Rausch S, Michael D, Kühl AA, Hartmann S
European journal of immunology 2017 Feb;47(2):353-363
European journal of immunology 2017 Feb;47(2):353-363
Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.
Zamarin D, Holmgaard RB, Ricca J, Plitt T, Palese P, Sharma P, Merghoub T, Wolchok JD, Allison JP
Nature communications 2017 Feb 13;8:14340
Nature communications 2017 Feb 13;8:14340
A Mouse Model of West Nile Virus Infection.
Graham JB, Swarts JL, Lund JM
Current protocols in mouse biology 2017 Dec 20;7(4):221-235
Current protocols in mouse biology 2017 Dec 20;7(4):221-235
Sex impacts Th1 cells, Tregs, and DCs in both intestinal and systemic immunity in a mouse strain and location-dependent manner.
Elderman M, van Beek A, Brandsma E, de Haan B, Savelkoul H, de Vos P, Faas M
Biology of sex differences 2016;7:21
Biology of sex differences 2016;7:21
Clonal expansion of CD4(+) cytotoxic T lymphocytes in patients with IgG4-related disease.
Mattoo H, Mahajan VS, Maehara T, Deshpande V, Della-Torre E, Wallace ZS, Kulikova M, Drijvers JM, Daccache J, Carruthers MN, Castelino FV, Stone JR, Stone JH, Pillai S
The Journal of allergy and clinical immunology 2016 Sep;138(3):825-838
The Journal of allergy and clinical immunology 2016 Sep;138(3):825-838
CD4(+) T-cell survival in the GI tract requires dectin-1 during fungal infection.
Drummond RA, Dambuza IM, Vautier S, Taylor JA, Reid DM, Bain CC, Underhill DM, Masopust D, Kaplan DH, Brown GD
Mucosal immunology 2016 Mar;9(2):492-502
Mucosal immunology 2016 Mar;9(2):492-502
B-cell anergy induces a Th17 shift in a novel B lymphocyte transgenic NOD mouse model, the 116C-NOD mouse.
Carrascal J, Carrillo J, Arpa B, Egia-Mendikute L, Rosell-Mases E, Pujol-Autonell I, Planas R, Mora C, Mauricio D, Ampudia RM, Vives-Pi M, Verdaguer J
European journal of immunology 2016 Mar;46(3):593-608
European journal of immunology 2016 Mar;46(3):593-608
Treatment with cyclophosphamide supported by various dendritic cell-based vaccines induces diversification in CD4⁺ T cell response against MC38 colon carcinoma.
Wojas-Turek J, Szczygieł A, Kicielińska J, Rossowska J, Piasecki E, Pajtasz-Piasecka E
International journal of oncology 2016 Feb;48(2):493-505
International journal of oncology 2016 Feb;48(2):493-505
Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.
Younis RH, Han KL, Webb TJ
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1419-29
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1419-29
Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation.
Cuff AO, Robertson FP, Stegmann KA, Pallett LJ, Maini MK, Davidson BR, Male V
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4283-4291
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4283-4291
Oxygen Sensing by T Cells Establishes an Immunologically Tolerant Metastatic Niche.
Clever D, Roychoudhuri R, Constantinides MG, Askenase MH, Sukumar M, Klebanoff CA, Eil RL, Hickman HD, Yu Z, Pan JH, Palmer DC, Phan AT, Goulding J, Gattinoni L, Goldrath AW, Belkaid Y, Restifo NP
Cell 2016 Aug 25;166(5):1117-1131.e14
Cell 2016 Aug 25;166(5):1117-1131.e14
Protein Translation Activity: A New Measure of Host Immune Cell Activation.
Seedhom MO, Hickman HD, Wei J, David A, Yewdell JW
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1498-506
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1498-506
Intranasal Administration of Lentiviral miR-135a Regulates Mast Cell and Allergen-Induced Inflammation by Targeting GATA-3.
Deng YQ, Yang YQ, Wang SB, Li F, Liu MZ, Hua QQ, Tao ZZ
PloS one 2015;10(9):e0139322
PloS one 2015;10(9):e0139322
Virulence-Dependent Alterations in the Kinetics of Immune Cells during Pulmonary Infection by Mycobacterium tuberculosis.
Kim WS, Kim JS, Cha SB, Han SJ, Kim H, Kwon KW, Kim SJ, Eum SY, Cho SN, Shin SJ
PloS one 2015;10(12):e0145234
PloS one 2015;10(12):e0145234
Triclosan Induces Thymic Stromal Lymphopoietin in Skin Promoting Th2 Allergic Responses.
Marshall NB, Lukomska E, Long CM, Kashon ML, Sharpnack DD, Nayak AP, Anderson KL, Jean Meade B, Anderson SE
Toxicological sciences : an official journal of the Society of Toxicology 2015 Sep;147(1):127-39
Toxicological sciences : an official journal of the Society of Toxicology 2015 Sep;147(1):127-39
Reciprocal regulation of lymphoid tissue development in the large intestine by IL-25 and IL-23.
Donaldson DS, Bradford BM, Artis D, Mabbott NA
Mucosal immunology 2015 May;8(3):582-95
Mucosal immunology 2015 May;8(3):582-95
Th17-type cytokines, IL-6 and TNF-α synergistically activate STAT3 and NF-kB to promote colorectal cancer cell growth.
De Simone V, Franzè E, Ronchetti G, Colantoni A, Fantini MC, Di Fusco D, Sica GS, Sileri P, MacDonald TT, Pallone F, Monteleone G, Stolfi C
Oncogene 2015 Jul;34(27):3493-503
Oncogene 2015 Jul;34(27):3493-503
The transduction pattern of IL-12-encoding lentiviral vectors shapes the immunological outcome.
Goyvaerts C, Broos K, Escors D, Heirman C, Raes G, De Baetselier P, Thielemans K, Breckpot K
European journal of immunology 2015 Dec;45(12):3351-61
European journal of immunology 2015 Dec;45(12):3351-61
CD4CD8αα lymphocytes, a novel human regulatory T cell subset induced by colonic bacteria and deficient in patients with inflammatory bowel disease.
Sarrabayrouse G, Bossard C, Chauvin JM, Jarry A, Meurette G, Quévrain E, Bridonneau C, Preisser L, Asehnoune K, Labarrière N, Altare F, Sokol H, Jotereau F
PLoS biology 2014 Apr;12(4):e1001833
PLoS biology 2014 Apr;12(4):e1001833
MyD88 signaling in CD4 T cells promotes IFN-γ production and hematopoietic progenitor cell expansion in response to intracellular bacterial infection.
Zhang Y, Jones M, McCabe A, Winslow GM, Avram D, MacNamara KC
Journal of immunology (Baltimore, Md. : 1950) 2013 May 1;190(9):4725-35
Journal of immunology (Baltimore, Md. : 1950) 2013 May 1;190(9):4725-35
NK cell maturation and function in C57BL/6 mice are altered by caloric restriction.
Clinthorne JF, Beli E, Duriancik DM, Gardner EM
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 15;190(2):712-22
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 15;190(2):712-22
Th17 effector cells support B cell responses outside of germinal centres.
Patakas A, Benson RA, Withers DR, Conigliaro P, McInnes IB, Brewer JM, Garside P
PloS one 2012;7(11):e49715
PloS one 2012;7(11):e49715
Increase in Th17 and T-reg lymphocytes and decrease of IL22 correlate with the recovery phase of acute EAE in rat.
Almolda B, Costa M, Montoya M, González B, Castellano B
PloS one 2011;6(11):e27473
PloS one 2011;6(11):e27473
CD134 plus CD137 dual costimulation induces Eomesodermin in CD4 T cells to program cytotoxic Th1 differentiation.
Qui HZ, Hagymasi AT, Bandyopadhyay S, St Rose MC, Ramanarasimhaiah R, Ménoret A, Mittler RS, Gordon SM, Reiner SL, Vella AT, Adler AJ
Journal of immunology (Baltimore, Md. : 1950) 2011 Oct 1;187(7):3555-64
Journal of immunology (Baltimore, Md. : 1950) 2011 Oct 1;187(7):3555-64
Cytokine requirements for the differentiation and expansion of IL-17A- and IL-22-producing human Vgamma2Vdelta2 T cells.
Ness-Schwickerath KJ, Jin C, Morita CT
Journal of immunology (Baltimore, Md. : 1950) 2010 Jun 15;184(12):7268-80
Journal of immunology (Baltimore, Md. : 1950) 2010 Jun 15;184(12):7268-80
Differential expression of T-bet, a T-box transcription factor required for Th1 T-cell development, in peripheral T-cell lymphomas.
Dorfman DM, van den Elzen P, Weng AP, Shahsafaei A, Glimcher LH
American journal of clinical pathology 2003 Dec;120(6):866-73
American journal of clinical pathology 2003 Dec;120(6):866-73
A novel transcription factor, T-bet, directs Th1 lineage commitment.
Szabo SJ, Kim ST, Costa GL, Zhang X, Fathman CG, Glimcher LH
Cell 2000 Mar 17;100(6):655-69
Cell 2000 Mar 17;100(6):655-69
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Supportive validation
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- Intracellular staining of normal human peripheral blood cells with Anti-Human CD8a APC (Product # 17-0086-42) and 0.06 µg of Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or 0.06 µg of Anti-Human/Mouse T-bet PE-Cyanine7 (right) using the Foxp3 Fixation/Permeabilization Buffers (Product # 00-5521-00). Cells in the lymphocyte gate were used for analysis.
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- Figure 2 1 wk ABX gvg-treated APP SWE /PS1 DeltaE9 mice display altered peripheral and brain inflammatory profiles. ( A ) Representative density dot plots of T-bet and Foxp3 intracellular expression in TCRbeta + CD4 + T cell populations isolated from MLN, blood and brain tissue of vehicle and 1 wk ABX gvg-treated APP SWE /PS1 DeltaE9 mice analysed by flow cytometry. Quantified percentages of ( B ) Foxp3 + and ( C ) T-bet + CD4 + T cells, representative of a T-reg and Th1 T cell phenotype respectively, are expressed relative to total live CD4 + T cell counts ( n = 5-6, *p < 0.05, **p < 0.01, un-paired two-tailed Student's t -test). ( D ) Immunoblot-based array of inflammatory mediators present in the serum of vehicle and 1 wk ABX gvg-treated APP SWE /PS1 DeltaE9 mice ( n = 10 pooled sera). ( E ) Immunoblot-based array of inflammatory mediators present in the CSF of vehicle and 1 wk ABX gvg-treated APP SWE /PS1 DeltaE9 mice ( n = 10 pooled CSF). ( F ) Heat map analysis of inflammatory mediator fold change expression in 1 wk ABX gvg-treated APP SWE /PS1 DeltaE9 mice relative to control. Data are displayed as log e (mean) or mean +- SEM. See Supp. Figs 2 , 4 , 5 , 6 , and statistical Table 2 for additional information.
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- Fig 5 Lentiviral-mmu-miR-135a treatment influences Th cell polarization. The expression of T-bet and GATA-3 protein in CD4 + T cells was measured in the spleens of normal (control), AR (AR-induced), positive (AR-induced, treated with lentiviral-mmu-miR-135a), and negative (AR-induced, treated with empty lentivirus) mice using flow cytometry. (A) Representative dot plots from each experimental group. The percentages of CD4 + T-bet + T cells (B) and CD4 + GATA-3 + T cells (C) were also calculated. Data are presented as the mean +- SEM. *P
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- Figure 3 Dynamics of Th1 cell population. A) Representative dot-plots of the population of CD4+Tbet+ cells of EAE animals. Dot-plots were obtained by previously gating in the CD3+ T cell population. Different quadrants were defined by application of the appropriate isotype control. A minimum of three animals per group was pooled and three replicates per score were analyzed. B and C) Histogrammes showing, respectively, the values corresponding to the total number and the percentage of CD4+Tbet+ cell population along EAE. Note that CD4+Tbet+ lymphocytes are found during the induction and peak phases and markedly decreased at score 2R of the recovery phase (ANOVA and Tukey's post-hoc test, *p
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- Fig. 9 In vivo administration of dNP2-siChi3l1 complex inhibits pulmonary metastasis with enhanced Th1 and CTL effector molecules. a Experimental scheme of dNP2-siRNA complex treatment in pulmonary melanoma metastasis model. b Representative lung image of dNP2-siEGFP and dNP2-siChi3l1-treated mice. Scale bar, 2 mm c Number of pleural colonies in each lung was counted. Data are mean +- SEM of three sets of independent experiments and each dot in graphs represent an individual mouse. d Relative total tumor area in the lung was measured by Image J software 1.48 v. e Histology of lung sections by H&E staining, and infiltrated tumor region was measured by Image J software 1.48 v. Scale bar, 200 m f , g IFNgamma producing CD4 and CD8 T cells, and Granzyme B expression level in IFNgamma + CD8 T cells in the lung was analyzed by intracellular staining. % of IFNgamma + , and MFI of Granzyme B was represented as scattered graph. h Intracellular T-bet expression level in CD4 + IFNgamma + and CD8 + IFNgamma + population. MFI was represented as scattered graph. i mRNA expression of genes related to cytotoxicity and Th1 effector functions was analyzed by quantitative RT-PCR. Each gene expression level was normalized to beta-actin. Data are mean +- SD of three sets of independent experiments and each dot in graphs represent an individual mouse. n.s., not significant; * p < 0.05, ** p < 0.01, *** p < 0.001 (two-tailed Student's t -test)
