Antibody data

Product number
48-5825-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
T-bet Monoclonal Antibody (eBio4B10 (4B10)), eFluor™ 450, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells. Applications Reported: This eBio4B10 antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio4B10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at www.thermofisher.com/flowprotocols . This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor 450 is an alternative to Pacific Blue. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Reactivity
Human, Mouse
Host
Mouse
Isotype
IgG
Antibody clone number
eBio4B10 (4B10)
Vial size
100 µg
Concentration
0.2 mg/mL
Storage
4°C, store in dark, DO NOT FREEZE!
TBX21 protein structure - 48-5825-82 shown in red.