Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB5385 - Provider product page
- Provider
- R&D Systems
- Product name
- Human T-bet/TBX21 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human T-bet.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9UL17
- Isotype
- IgG
- Antibody clone number
- 525803
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The effect of adalimumab on key drivers in the pathogenesis of psoriasis.
TLR2 stimulation drives human naive and effector regulatory T cells into a Th17-like phenotype with reduced suppressive function.
Regional immunity in melanoma: immunosuppressive changes precede nodal metastasis.
Hendriks AG, van der Velden HM, Wolberink EA, Seyger MM, Schalkwijk J, Zeeuwen PL, de Jong EM, Pasch MC, van Erp PE, van de Kerkhof PC
The British journal of dermatology 2014 Mar;170(3):571-80
The British journal of dermatology 2014 Mar;170(3):571-80
TLR2 stimulation drives human naive and effector regulatory T cells into a Th17-like phenotype with reduced suppressive function.
Nyirenda MH, Sanvito L, Darlington PJ, O'Brien K, Zhang GX, Constantinescu CS, Bar-Or A, Gran B
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 1;187(5):2278-90
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 1;187(5):2278-90
Regional immunity in melanoma: immunosuppressive changes precede nodal metastasis.
Mansfield AS, Holtan SG, Grotz TE, Allred JB, Jakub JW, Erickson LA, Markovic SN
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 Apr;24(4):487-94
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 Apr;24(4):487-94
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human T-bet/TBX21 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line . PVDF membrane was probed with 2 µg/mL of Human T-bet/TBX21 Monoclonal Antibody (Catalog # MAB5385) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for T-bet/TBX21 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of T-bet/TBX21 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Human T-bet/TBX21 Monoclonal Antibody (Catalog # MAB5385, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with ice-cold methanol.