15280-1-AP

antibody from Proteintech Group

Targeting: ATP6V1E1 ATP6E, ATP6E2, ATP6V1E, P31, Vma4

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

15280-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#15280-1-AP, RRID:AB_2062545

Product name

ATP6V1E1 antibody

Antibody type

Polyclonal

Description

KD/KO validated ATP6V1E1 antibody (Cat. #15280-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Exploring the mechanism and phytochemicals in Psoraleae Fructus-induced hepatotoxicity based on RNA-seq, in vitro screening and molecular docking.

Shang H, Liu X, Pan J, Cheng H, Ma Z, Xiao C, Gao Y
Scientific reports 2024 Jan 19;14(1):1696

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Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification.

Choezom D, Gross JC
Journal of cell science 2022 Mar 1;135(5)

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Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly.

Ratto E, Chowdhury SR, Siefert NS, Schneider M, Wittmann M, Helm D, Palm W
Nature communications 2022 Aug 17;13(1):4848

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Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.

Yuan L, Chu B, Chen S, Li Y, Liu N, Zhu Y, Zhou D
Microorganisms 2021 Nov 16;9(11)

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Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.

Chu B, Zhu Y, Su J, Xia B, Zou Y, Nie J, Zhang W, Wang J
Veterinary research 2020 Aug 5;51(1):99

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iTRAQ-based quantitative proteomic analysis for identification of oligodendroglioma biomarkers related with loss of heterozygosity on chromosomal arm 1p.

Yang LS, Xu XE, Liu XP, Jin H, Chen ZQ, Liu XH, Wang Y, Huang FP, Shi Q
Journal of proteomics 2012 Dec 21;77:480-91

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Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification.

Wong D, Bach H, Sun J, Hmama Z, Av-Gay Y
Proceedings of the National Academy of Sciences of the United States of America 2011 Nov 29;108(48):19371-6

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human brain tissue were subjected to SDS PAGE followed by western blot with 15280-1-AP(ATP6V1E1 antibody) at dilution of 1:500

Sample type

tissue

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-ATP6V1E1 (IP:15280-1-AP, 3ug; Detection:15280-1-AP 1:500) with mouse brain tissue lysate 6000ug.

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The ATP6V1E1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ATP6V1E1. This antibody recognizes human,mouse,rat antigen. The ATP6V1E1 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.

Appendix

15280-1-AP.pdf

Show more

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 15280-1-AP(ATP6V1E1 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue