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Western blot
Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00000529-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000529-M02, RRID:AB_11013239
- Product name
- Mouse Monoclonal ATP6V1E1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Atg5 Disassociates the V1V0-ATPase to Promote Exosome Production and Tumor Metastasis Independent of Canonical Macroautophagy.
Guo H, Chitiprolu M, Roncevic L, Javalet C, Hemming FJ, Trung MT, Meng L, Latreille E, Tanese de Souza C, McCulloch D, Baldwin RM, Auer R, Côté J, Russell RC, Sadoul R, Gibbings D
Developmental cell 2017 Dec 18;43(6):716-730.e7
Developmental cell 2017 Dec 18;43(6):716-730.e7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATP6V1E1 Antibody (4E11) [H00000529-M02] - Analysis of ATP6V1E1 expression in transfected 293T cell line by ATP6V1E1 monoclonal antibody (M02), clone 4E11.Lane 1: ATP6V1E1 transfected lysate(26.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ATP6V1E1 Antibody (4E11) [H00000529-M02] - Detection limit for recombinant GST tagged ATP6V1E1 is 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ATP6V1E1 Antibody (4E11) [H00000529-M02] - Analysis of monoclonal antibody to ATP6V1E1 on HeLa cell . Antibody concentration 10 ug/ml.
