Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- Ab090180 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- ATP6V1E1 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit anti Human ATP6V1E1 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.Tissue specificity: Ubiquitous.Similarity: Belongs to the V-ATPase E subunit family.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 20-226- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,6 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
No comments: Submit comment
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ATP6V1E1 Antibody (Ab090180) - Western Blot All lanes: ATP6V1E1 Antibody (Ab090180) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 26 kDa Observed band size: 28 kDa Exposure time: 53.1 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ATP6V1E1 Antibody (Ab090180) - IF/ICC Immunocytochemistry analysis of ATP6V1E1 (green) in Hela using ATP6V1E1 Antibody (Ab090180), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ATP6V1E1 Antibody (Ab090180) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using ATP6V1E1 Antibody (Ab090180). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
