TA502322

antibody from OriGene

Targeting: ATP6V1F ATP6S14, VATF, Vma7

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502322

Provider

OriGene

Proper citation

OriGene Cat#TA502322, RRID:AB_11139712

Product name

ATP6V1F mouse monoclonal antibody, clone OTI6C11 (formerly 6C11)

Antibody type

Monoclonal

Description

ATP6V1F mouse monoclonal antibody, clone OTI6C11 (formerly 6C11)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

ATP6V1F

Isotype

IgG

Antibody clone number

OTI6C11

Vial size

100 µl

Concentration

0.44 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1F (RC210728, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1F.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP6V1F monoclonal antibody.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC210728 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ATP6V1F antibody(TA502322), and then analyzed by flow cytometry.

Validation comment

FC