MA5-25408

antibody from Invitrogen Antibodies

Targeting: ATP6V1F ATP6S14, VATF, Vma7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

MA5-25408

Provider

Invitrogen Antibodies

Product name

ATP6V1F Monoclonal Antibody (OTI5F10)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

OTI5F10

Vial size

100 µL

Concentration

0.83 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ATP6V1F in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cells using 35 µg per lane. Samples were separated by SDS-PAGE and probed with ATP6V1F (Product # MA5-25408) monoclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ATP6V1F in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with ATP6V1F (Product # MA5-25408) monoclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ATP6V1F in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cells using 35 µg per lane. Samples were separated by SDS-PAGE and probed with ATP6V1F (Product # MA5-25408) monoclonal antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ATP6V1F in COS7 cells. Cells were transfected with a plasmid overexpressing ATP6V1F and probed with a ATP6V1F monoclonal antibody (Product # MA5-25408).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ATP6V1F in COS7 cells. Cells were transfected with a plasmid overexpressing ATP6V1F and probed with a ATP6V1F monoclonal antibody (Product # MA5-25408).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ATP6V1F in COS7 cells. Cells were transfected with a plasmid overexpressing ATP6V1F and probed with a ATP6V1F monoclonal antibody (Product # MA5-25408).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ATP6V1F in COS7 cells. Cells were transfected with a plasmid overexpressing ATP6V1F and probed with a ATP6V1F monoclonal antibody (Product # MA5-25408).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human kidney tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a ATP6V1F monoclonal antibody (Product # MA5-25408).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human lymphoma tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a ATP6V1F monoclonal antibody (Product # MA5-25408).