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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB300-180 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-180, RRID:AB_2200581
- Product name
- Rabbit Polyclonal Synapsin I Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for ~78k synapsin I doublet protein phosphorylated at Ser9. The antibody also weakly labels the ~55k synapsin II protein which has a similar phosphorylation site to that of Ser9 on synapsin I. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with lambda phosphatase.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Synapsin I [p Ser9] Antibody [NB300-180] - Rat cortex lysate showing specific immunolabeling of the ~78k synapsin I phosphorylated at Ser9 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser9 synapsin I antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Synapsin I [p Ser9] Antibody [NB300-180] - Cultured mouse caudate neurons showing synapsin I when phosphorylated at Ser9
