Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB300-744-0.1ml - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-744-0.1ml, RRID:AB_10002135
- Product name
- Rabbit Polyclonal Synapsin I Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for ~78k synapsin I doublet phosphorylated at Ser549. Immunolabeling of the synapsin I band is blocked by lambda-phosphatase treatment.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Synapsin I [p Ser549] Antibody [NB300-744] - Western blot of rat brain lysate showing specific immunolabeling of the ~78k synapsin I phosphorylated at Ser549 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambdlambda-Ptase). The blot is identical ot the control except that it was incubated in lambdlambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho Ser549 synapsin I. The immunolabeling is completely eliminated by treatment with lambdlambda-Ptase.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Synapsin I [p Ser549] Antibody [NB300-744] - Immunostaining of cultured mouse caudate neurons showing synapsin I when hosphorylated at Ser549.