OPA1-04001

antibody from Invitrogen Antibodies

Targeting: SYN1 MRX50

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

OPA1-04001

Provider

Invitrogen Antibodies

Product name

Synapsin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Purifed from natural sources

Description

In Western blot, this antibody detects an ~77 kDa and ~80 kDa protein representing synapsin I alpha and synapsin I beta respectively, in rat brain homogenate. Immunohistochemical staining of synapsin 1 alpha and 1 beta in human brain with OPA1-04001 yields a pattern consistent with cytoplasmic vesicle staining.

Reactivity

Human, Mouse, Rat, Bovine

Host

Rabbit

Isotype

IgG

Vial size

50 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Hierarchical organization and genetically separable subfamilies of PSD95 postsynaptic supercomplexes.

Frank RAW, Zhu F, Komiyama NH, Grant SGN
Journal of neurochemistry 2017 Aug;142(4):504-511

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of Synapsin 1 in rat hippocampal lysate showing specific immunolabeling of a ~78 kDa band corresponding to Synapsin 1 polyclonal antibody (Product # OPA1-04001).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using anti-Synapsin1 Polyclonal Antibody (Product # OPA1-04001) and 78 kDa band corresponding to Synapsin1 was observed across cell lines and tissues tested except Mouse and Rat Lung, Mouse Heart and Raji which are reported to be negative. Whole cell Extracts of (30ug lysate) of IMR-32 (Lane 1), PC-12 (Lane 2), Raji (Lane 8), tissue extracts of Mouse Brain (Lane 3), Rat Brain (Lane 4), Mouse Lung (Lane 5), Rat Lung (Lane 6) and Mouse Heart (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Neuroanatomically restricted assembly of Kir2.3-N-methyl D-aspartic acid receptors ( NMDAR ) ion channel-channel supercomplexes. (a) Regional enrichment of Kir2.3 expression in mouse brain. Left, mouse brain sagittal sections (lower) stained with Kir2.3 antibodies and (upper) reference Nissl stain (Allen Brain Atlas). High expression in rostroventral midbrain and caudodorsal forebrain shown and boxed regions were dissected for TAP -purification of NMDAR . White arrow indicates boxed region of piriform cortex used in high-magnification images in Fig. 4b and c. Scale bar, 1 mm. Right, BNP immunoblots of TAP -purified receptors from dissected brain regions of Glun1 TAP / TAP mice as indicated. Left immunoblot probed with Flag ( TAP -GluN1) and right immunoblot probed with Kir2.3 showing 1.5-Kir2.3- NMDAR ion channel-channel supercomplex enriched in rostroventral midbrain. Filled arrow, 1.5-Kir2.3/ NR . Molecular weight in MD a shown on right. IB , immunoblotting antibody. (b) Kir2.3 localization in layers of piriform cortex of wildtype ( WT ) and Psd95 -/- mice, including layers 1a (L1a) and 2/3 (L2/3) of piriform cortex stained with antibodies to Kir2.3 (green) and nuclear stain ( DAPI , blue). White boxes indicate regions further magnified in Fig. 4c. Scale bar, 6 mum. (c) Kir2.3 localization requires Psd95 . Higher magnification of boxed regions in Fig. 4b shows synaptic localization of Kir2.3 is disrupted in Psd95 -/- mice. Sections double-stained with Kir2.3 (gre