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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-22750 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-22750, RRID:AB_2257984
- Product name
- Anti-ADH1C
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Mechanism of protection against alcoholism by an alcohol dehydrogenase polymorphism: development of an animal model.
Rivera-Meza M, Quintanilla ME, Tampier L, Mura CV, Sapag A, Israel Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Jan;24(1):266-74
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Jan;24(1):266-74
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 40
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
