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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- TA328614 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-proBDNF
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-proBDNF
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- BDNF
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
Submitted references Brain-derived neurotrophic factor-TrkB signaling in the medial prefrontal cortex plays a role in the anhedonia-like phenotype after spared nerve injury.
Fang X, Yang C, Li S, Zhan G, Zhang J, Huang N, Du X, Xu H, Hashimoto K, Luo A
European archives of psychiatry and clinical neuroscience 2018 Jun 7;
European archives of psychiatry and clinical neuroscience 2018 Jun 7;
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of rat glioma C6 (1, 3) or human neuroblastoma SH-SY5Y (2, 4) cell lysate: 1, 2. Anti-proBDNF antibody (1:200). 3, 4. Anti-proBDNF antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using Anti-proBDNF antibody, (1:400): 1. proBDNF (WT-human), (20 ng). 2. proNGF (WT-human), (200 ng). 3. recombinant proNT-3 (200 ng). 4. hBDNF , (200 ng). 5. mNGF 2.5S (Grade II), (200 ng). 6. hNT-3, (200 ng).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of proBDNF in rat cerebellum. Immunohistochemical staining of rat cerebellum with Anti-proBDNF antibody. proBDNF (red) appears in Purkinje cell bodies (vertical arrows) and in astrocytic processes (horizontal arrows in A) but not in Purkinje neuronal dendrites stained for calbindin D28k (green, in B) in the same brain section. C. Confocal merge of proBDNF and CBD28K demonstrates the restriction of proBDNF to Purkinje cell body.
- Validation comment
- IHC
