Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB2481 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal BDNF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human BDNF in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NT-3, rhNT-4, or rh beta-NGF is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references BDNF and its receptors in human myasthenic thymus: implications for cell fate in thymic pathology.
Berzi A, Ayata CK, Cavalcante P, Falcone C, Candiago E, Motta T, Bernasconi P, Hohlfeld R, Mantegazza R, Meinl E, Farina C
Journal of neuroimmunology 2008 Jul 15;197(2):128-39
Journal of neuroimmunology 2008 Jul 15;197(2):128-39
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of BDNF in U-87 MG Human Cell Line by Flow Cytometry. U-87 MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human BDNF Monoclonal Antibody (Catalog # MAB2481, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.