Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB648-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human BDNF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human BDNF in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) NT-3 or rhNT-4.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P23560
- Isotype
- IgG
- Antibody clone number
- 37141
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Neuroendocrine cells of nasal mucosa are a cellular source of brain-derived neurotrophic factor.
Jornot L, Lacroix JS, Rochat T
The European respiratory journal 2008 Sep;32(3):769-74
The European respiratory journal 2008 Sep;32(3):769-74
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human BDNF ELISA Standard Curve. Recombinant Human BDNF protein was serially diluted 2-fold and captured by Mouse Anti-Human BDNF Monoclonal Antibody (Catalog # MAB848R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human BDNF Monoclonal Antibody (Catalog # MAB648) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).