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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [5]
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- Product number
- LS-C483057 - Provider product page
- Provider
- LSBio
- Product name
- BDNF Antibody (aa20-70) LS-C483057
- Antibody type
- Polyclonal
- Description
- Antiserum
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Storage
- Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2°C to 8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to pro BDNF (20-70). WB on rat tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to pro BDNF (20-70). WB on human platelet lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody incubated at 4C overnight. Mature BDNF: OSB00032W (1:2000 dilution) ; proBDNF (1:1000 dilution)
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to pro BDNF (20-70). IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mm Hg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions, using DAB chromogen. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. Small neurons are stained and also some nuclear staining is observed.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to pro BDNF (20-70). IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mm Hg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions, using DAB chromogen. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. Small neurons are stained and also some nuclear staining is observed.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to pro BDNF (20-70). IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mm Hg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions, using DAB chromogen. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. Small neurons are stained and also some nuclear staining is observed.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to pro BDNF (20-70). IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mm Hg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions, using DAB chromogen. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. Small neurons are stained and also some nuclear staining is observed.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to pro BDNF (20-70). IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mm Hg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions, using DAB chromogen. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. Small neurons are stained and also some nuclear staining is observed.
