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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunohistochemistry [5]
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- Product number
- NBP1-46750 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal BDNF Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. Specific for mature BDNF.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- LYOPH
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Enriched Environment Reverts Somatostatin Interneuron Loss in MK-801 Model of Schizophrenia.
Hindbrain Estrogen Receptor Regulation of Ventromedial Hypothalamic Glycogen Metabolism and Glucoregulatory Transmitter Expression in the Hypoglycemic Female Rat.
Silencing of PHLPP1 promotes neuronal apoptosis and inhibits functional recovery after spinal cord injury in mice.
Sex differences in forebrain estrogen receptor regulation of hypoglycemic patterns of counter-regulatory hormone secretion and ventromedial hypothalamic nucleus glucoregulatory neurotransmitter and astrocyte glycogen metabolic enzyme expression.
Murueta-Goyena A, Ortuzar N, Lafuente JV, Bengoetxea H
Molecular neurobiology 2020 Jan;57(1):125-134
Molecular neurobiology 2020 Jan;57(1):125-134
Hindbrain Estrogen Receptor Regulation of Ventromedial Hypothalamic Glycogen Metabolism and Glucoregulatory Transmitter Expression in the Hypoglycemic Female Rat.
Napit PR, Ali MH, Shakya M, Mandal SK, Bheemanapally K, Mahmood ASMH, Ibrahim MMH, Briski KP
Neuroscience 2019 Jul 15;411:211-221
Neuroscience 2019 Jul 15;411:211-221
Silencing of PHLPP1 promotes neuronal apoptosis and inhibits functional recovery after spinal cord injury in mice.
Shao Z, Lv G, Wen P, Cao Y, Yu D, Lu Y, Li G, Su Z, Teng P, Gao K, Wang Y, Mei X
Life sciences 2018 Sep 15;209:291-299
Life sciences 2018 Sep 15;209:291-299
Sex differences in forebrain estrogen receptor regulation of hypoglycemic patterns of counter-regulatory hormone secretion and ventromedial hypothalamic nucleus glucoregulatory neurotransmitter and astrocyte glycogen metabolic enzyme expression.
Hasan Mahmood ASM, Uddin MM, Ibrahim MMH, Mandal SK, Alhamami HN, Briski KP
Neuropeptides 2018 Dec;72:65-74
Neuropeptides 2018 Dec;72:65-74
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BDNF Antibody [NBP1-46750] - Human platelet lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody incubated at 4C overnight. Click on eLAB BOOK tab to see the details. Mature BDNF: (1:2000 dilution) ; proBDNF: (1:1000 dilution)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - IHC-P on paraffin sections of rat hippocampus.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of mouse hippocampus. Dilution 1: 1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of rat spinal cord. Dilution 1: 1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of rat hippocampus. Dilution 1: 1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of rat hippocampus. Dilution 1: 1000.
