Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- M00035-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-BDNF Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for BDNF detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat BDNF information: Molecular Weight: 27818 MW; Subcellular Localization: Secreted; Tissue Specificity: Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- BEH-2
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Menthol-Modified Quercetin Liposomes with Brain-Targeting Function for the Treatment of Senescent Alzheimer's Disease.
Esketamine Prevents Postoperative Emotional and Cognitive Dysfunction by Suppressing Microglial M1 Polarization and Regulating the BDNF-TrkB Pathway in Ageing Rats with Preoperative Sleep Disturbance.
The hippocampal FTO-BDNF-TrkB pathway is required for novel object recognition memory reconsolidation in mice.
Biological effects of exposure to 2650 MHz electromagnetic radiation on the behavior, learning, and memory of mice.
Formononetin improves cardiac function and depressive behaviours in myocardial infarction with depression by targeting GSK-3β to regulate macrophage/microglial polarization.
Soluble dietary fiber and cellulose from Saccharina japonica by-product ameliorate Loperamide-induced constipation via modulating enteric neurotransmitters, short-chain fatty acids and gut microbiota.
SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo.
Fluoride Stimulates Anxiety- and Depression-like Behaviors Associated with SIK2-CRTC1 Signaling Dysfunction.
Effects of combination treatment with transcranial magnetic stimulation and bone marrow mesenchymal stem cell transplantation or Raf inhibition on spinal cord injury in rats.
Differential expression of microRNAs in TM3 Leydig cells of mice treated with brain-derived neurotrophic factor.
Protocatechuic acid improves cognitive deficits and attenuates amyloid deposits, inflammatory response in aged AβPP/PS1 double transgenic mice.
Curcumin improves spatial memory impairment induced by human immunodeficiency virus type 1 glycoprotein 120 V3 loop peptide in rats.
Application of bFGF and BDNF to improve angiogenesis and cardiac function.
Moclobemide up-regulates proliferation of hippocampal progenitor cells in chronically stressed mice.
Liu WY, Yu Y, Zang J, Liu Y, Li FR, Zhang L, Guo RB, Kong L, Ma LY, Li XT
ACS chemical neuroscience 2024 Jun 5;15(11):2283-2295
ACS chemical neuroscience 2024 Jun 5;15(11):2283-2295
Esketamine Prevents Postoperative Emotional and Cognitive Dysfunction by Suppressing Microglial M1 Polarization and Regulating the BDNF-TrkB Pathway in Ageing Rats with Preoperative Sleep Disturbance.
Wen Y, Xu J, Shen J, Tang Z, Li S, Zhang Q, Li J, Sun J
Molecular neurobiology 2024 Aug;61(8):5680-5698
Molecular neurobiology 2024 Aug;61(8):5680-5698
The hippocampal FTO-BDNF-TrkB pathway is required for novel object recognition memory reconsolidation in mice.
Chang R, Zhu S, Peng J, Lang Z, Zhou X, Liao H, Zou J, Zeng P, Tan S
Translational psychiatry 2023 Nov 14;13(1):349
Translational psychiatry 2023 Nov 14;13(1):349
Biological effects of exposure to 2650 MHz electromagnetic radiation on the behavior, learning, and memory of mice.
Zheng R, Zhang X, Gao Y, Gao D, Gong W, Zhang C, Dong G, Li Z
Brain and behavior 2023 Jun;13(6):e3004
Brain and behavior 2023 Jun;13(6):e3004
Formononetin improves cardiac function and depressive behaviours in myocardial infarction with depression by targeting GSK-3β to regulate macrophage/microglial polarization.
Yang Y, Huang T, Zhang H, Li X, Shi S, Tian X, Huang Z, Zhang R, Liu Z, Cheng Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 Jan;109:154602
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 Jan;109:154602
Soluble dietary fiber and cellulose from Saccharina japonica by-product ameliorate Loperamide-induced constipation via modulating enteric neurotransmitters, short-chain fatty acids and gut microbiota.
Cao J, Wang K, Li N, Zhang L, Qin L, He Y, Wang J, Qu C, Miao J
International journal of biological macromolecules 2023 Jan 31;226:1319-1331
International journal of biological macromolecules 2023 Jan 31;226:1319-1331
SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo.
Wang R, Yang M, Wu Y, Liu R, Liu M, Li Q, Su X, Xin Y, Huo W, Deng Q, Ba Y, Huang H
International journal of biological macromolecules 2022 Sep 30;217:219-228
International journal of biological macromolecules 2022 Sep 30;217:219-228
Fluoride Stimulates Anxiety- and Depression-like Behaviors Associated with SIK2-CRTC1 Signaling Dysfunction.
Zhou G, Hu Y, Wang A, Guo M, Du Y, Gong Y, Ding L, Feng Z, Hou X, Xu K, Yu F, Li Z, Ba Y
Journal of agricultural and food chemistry 2021 Nov 17;69(45):13618-13627
Journal of agricultural and food chemistry 2021 Nov 17;69(45):13618-13627
Effects of combination treatment with transcranial magnetic stimulation and bone marrow mesenchymal stem cell transplantation or Raf inhibition on spinal cord injury in rats.
Feng S, Wang S, Sun S, Su H, Zhang L
Molecular medicine reports 2021 Apr;23(4)
Molecular medicine reports 2021 Apr;23(4)
Differential expression of microRNAs in TM3 Leydig cells of mice treated with brain-derived neurotrophic factor.
Gao S, Li C, Xu Y, Chen S, Zhao Y, Chen L, Jiang Y, Liu Z, Fan R, Sun L, Wang F, Zhu X, Zhang J, Zhou X
Cell biochemistry and function 2017 Oct;35(7):364-371
Cell biochemistry and function 2017 Oct;35(7):364-371
Protocatechuic acid improves cognitive deficits and attenuates amyloid deposits, inflammatory response in aged AβPP/PS1 double transgenic mice.
Song Y, Cui T, Xie N, Zhang X, Qian Z, Liu J
International immunopharmacology 2014 May;20(1):276-81
International immunopharmacology 2014 May;20(1):276-81
Curcumin improves spatial memory impairment induced by human immunodeficiency virus type 1 glycoprotein 120 V3 loop peptide in rats.
Tang H, Lu D, Pan R, Qin X, Xiong H, Dong J
Life sciences 2009 Jul 3;85(1-2):1-10
Life sciences 2009 Jul 3;85(1-2):1-10
Application of bFGF and BDNF to improve angiogenesis and cardiac function.
Liu Y, Sun L, Huan Y, Zhao H, Deng J
The Journal of surgical research 2006 Nov;136(1):85-91
The Journal of surgical research 2006 Nov;136(1):85-91
Moclobemide up-regulates proliferation of hippocampal progenitor cells in chronically stressed mice.
Li YF, Zhang YZ, Liu YQ, Wang HL, Yuan L, Luo ZP
Acta pharmacologica Sinica 2004 Nov;25(11):1408-12
Acta pharmacologica Sinica 2004 Nov;25(11):1408-12
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of Human cerebellum lysate, using BDNF antibody (M00035-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BDNF monoclonal antibody (Catalog # M00035-1) overnight at 4 °C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BDNF
- Additional image