ANT-006-25UL

antibody from Invitrogen Antibodies

Targeting: BDNF

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ANT-006-25UL

Provider

Invitrogen Antibodies

Product name

proBDNF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysisof rat glioma C6 (1, 3) or human neuroblastoma SH-SY5Y (2, 4) cell lysate: - 1,2. Anti-proBDNF Antibody (#ANT-006), (1:200).3,4. Anti-proBDNF Antibody , preincubated with proBDNF Blocking Peptide (#BLP-NT006).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using Anti-proBDNF Antibody (#ANT-006), (1:400): - 1.Recombinant human proBDNF protein (#B-257), (20 ng).2.Recombinant human proNGF protein (#N-280), (200 ng).3. Recombinant proNT-3 (200 ng).4. Recombinanthuman BDNF protein (#B-250), (200 ng).5.Native mouse NGF 2.5S protein (>95%) (#N-100), (200 ng).6.Recombinant human Neurotrophin-3 (NT-3) protein (#N-260), (200 ng).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysisof rat glioma C6 (1, 3) or human neuroblastoma SH-SY5Y (2, 4) cell lysate: - 1,2. Anti-proBDNF Antibody (#ANT-006), (1:200).3,4. Anti-proBDNF Antibody , preincubated with proBDNF Blocking Peptide (#BLP-NT006).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using Anti-proBDNF Antibody (#ANT-006), (1:400): - 1.Recombinant human proBDNF protein (#B-257), (20 ng).2.Recombinant human proNGF protein (#N-280), (200 ng).3. Recombinant proNT-3 (200 ng).4. Recombinanthuman BDNF protein (#B-250), (200 ng).5.Native mouse NGF 2.5S protein (>95%) (#N-100), (200 ng).6.Recombinant human Neurotrophin-3 (NT-3) protein (#N-260), (200 ng).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of proBDNF in rat cerebellum - Immunohistochemical staining of rat cerebellum with Anti-proBDNF Antibody (#ANT-006). proBDNF (red) appears in Purkinje cell bodies (vertical arrows) and in astrocytic processes (horizontal arrows in A) but not in Purkinje neuronal dendrites stained for calbindin D28k (green, in B) in the same brain section. C. Confocal merge of proBDNF and CBD28K demonstrates the restriction of proBDNF to Purkinje cell body.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Multiplex staining of BDNF and proBDNF in rat hippocampus - Immunohistochemical staining of rat hippocampal dentate gyrus perfusion-fixed frozen sections using Guinea pig Anti-BDNF Antibody (#ANT-010-GP), (1:300) and Anti-proBDNF Antibody (#ANT-006), (1:200). A. BDNF staining (red) appears in interneuron outlines (arrows) in the hilus (H) region and in the granule layer (G). B. proBDNF staining (green) in the same section appears in interneuron outlines (arrows) in the hilus (H) region and in the granule layer (G). C. Merge of panel A and panel B shows extensive co-localization of the two proteins.