- OSB00017W - Invitrogen Antibodies BDNF antibody

Submitted references Exosomal miR-29b of Gut Origin in Patients With Ulcerative Colitis Suppresses Heart Brain-Derived Neurotrophic Factor.

Lian H, Zhong XS, Xiao Y, Sun Z, Shen Y, Zhao K, Ma X, Li Y, Niu Q, Liu M, Powell DW, Liu C, Li Q
Frontiers in molecular biosciences 2022;9:759689

Chronic colitis upregulates microRNAs suppressing brain-derived neurotrophic factor in the adult heart.

Tang Y, Kline KT, Zhong XS, Xiao Y, Lian H, Peng J, Liu X, Powell DW, Tang G, Li Q
PloS one 2021;16(9):e0257280

Administration of Bifidobacterium bifidum BGN4 and Bifidobacterium longum BORI Improves Cognitive and Memory Function in the Mouse Model of Alzheimer's Disease.

Kim H, Kim S, Park SJ, Park G, Shin H, Park MS, Kim J
Frontiers in aging neuroscience 2021;13:709091

Effects of Radix Polygalae on Cognitive Decline and Depression in Estradiol Depletion Mouse Model of Menopause.

Han G, Choi J, Cha SY, Kim BI, Kho HK, Jang MJ, Kim MA, Maeng S, Hong H
Current issues in molecular biology 2021 Oct 19;43(3):1669-1684

Predicting neuroblastoma using developmental signals and a logic-based model.

Kasemeier-Kulesa JC, Schnell S, Woolley T, Spengler JA, Morrison JA, McKinney MC, Pushel I, Wolfe LA, Kulesa PM
Biophysical chemistry 2018 Jul;238:30-38

Maternal low-protein diet decreases brain-derived neurotrophic factor expression in the brains of the neonatal rat offspring.

Marwarha G, Claycombe-Larson K, Schommer J, Ghribi O
The Journal of nutritional biochemistry 2017 Jul;45:54-66

Editing DNA Methylation in the Mammalian Genome.

Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R
Cell 2016 Sep 22;167(1):233-247.e17

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on human platelet lysate and recombinant mature BDNF using BDNF Polyclonal Antibody (Product #OSB00017W). Blocking: 1% LFDM for 30 min at RT; primary antibody (1:1000) incubated at 4C overnight.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot was performed using Anti-BDNF Polyclonal Antibody (Product # OSB00017W) and a 30 kDa band corresponding to BDNF was observed across tissues (Fig. a) and cell lines (Fig. b). Whole cell extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Spleen (Lane 3), Mouse Liver (Lane 4) in Fig. a and U-87 MG (Lane 1), A549 (Lane 2), MDA-MB-231 (Lane 3), Hep G2 (Lane 4) and MOLT-4 (Lane 5) in Fig. b were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band (*) was observed at around 45 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of BDNF using a polyclonal antibody (Product # OSB00017W).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of BDNF using a polyclonal antibody (Product # OSB00017W).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of BDNF using a polyclonal antibody (Product # OSB00017W).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis was performed in sections of mouse hippocampus using BDNF Polyclonal Antibody (Product #OSB00017W). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis was performed in sections of mouse hippocampus using BDNF Polyclonal Antibody (Product #OSB00017W). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis was performed in sections of mouse hippocampus using BDNF Polyclonal Antibody (Product #OSB00017W). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 B. bifidum BGN4 and B. longum BORI reduced Alzheimer's disease (AD) pathogenesis in the 5xFAD Mouse Brain. (A) Representative images of Map2 and BDNF positive cells in the CA3 hippocampal subfields of control or 5xFAD mice treated with B. bifidum BGN4 and B. longum BORI (BGN4/BORI). Scale bar = 50 mum. (B) Quantification of the Map2+/BDNF+ neurons in the CA3 hippocampal region at 30 days. Data represent the mean +- SEM. ANOVA , * p < 0.05, ** p < 0.01 ( n = 5). (C) Western blot analysis of the scaffolding proteins, PSD95 and Homer1, and blood brain-derived neurotrophic factor, BDNF, in the hippocampus of control or 5xFAD mice treated with B. bifidum BGN4 and B. longum BORI. (D) Hippocampal quantification of BDNF expression were normalized to beta-actin. Data represent the mean +- SEM. ANOVA , * p < 0.05, ** p < 0.01 ( n = 5). (E) Hippocampal quantifications of PSD95 (left) and Homer1 (right) expression were normalized to beta-actin. Data represent the mean +- SEM. ANOVA , ** p < 0.01 ( n = 5). (F) Representative images showing the production of amyloid-beta42 (top) and cleaved-caspase3 (bottom) in the CA3 hippocampal subfields of control or 5xFAD mice treated with B. bifidum BGN4 and B. longum BORI. Scale bar = 20 mum. (G,H) Quantifications of the amyloid-beta42+ (G) and cleaved-caspase3+ (H) cells in the CA3 hippocampal region at 30 days. Data represent the mean +- SEM. ANOVA , * p < 0.05, ** p < 0.01 ( n = 6). (I) Immunodetection of amyloid precursor protein (APP),

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: 10.1371/journal.pone.0257280.g003 Fig 3 miR-1b, Let-7d, and miR-155 suppress BDNF in H9c2 cells. Mimics of miR-1b, Let-7d, and miR-155 or their combination were overexpressed in H9c2 cells by transient transfection. Top panel: Western blots showing miR-1b, Let-7d, and miR-155-5p, either alone or in combination, suppressed BDNF protein expression. Bar graph: Relative band density normalized to the housekeeping gene GAPDH. n = 3 independent experiment. * p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: FIGURE 7 Exosomes rich in miR-29b suppress BDNF and enhance cleaved caspase 3 in vitro (cell culture) and in vivo (mouse tail vein injection). (A) The medium from IL-1beta-treated CCD841 CoN cells suppressed BDNF mRNA (left panel) and protein levels (right panel) in H9c2 cells. CCD841 CoN cells were transfected with miR-29b inhibitor (inh) and treated with 10 ng/ml IL-1beta. The medium without IL-1beta was collected and used to treat H9c2 cells for 24 h. (B) Representative images of Western blots showing exosomal miR-29b decreased BDNF in vitro and in vivo . Bar graphs show the relative band densities of BDNF (C) and cleaved caspase 3 (D) after normalization to the housekeeping gene GAPDH. n = 3 independent experiments for H9c2 cells. n = 5 for in vivo experiments. * p < 0.01 vs. controls. CCD841 CoN-derived or mouse plasma exosomes were transfected with miR-29b or control miRNA. The CCD841 CoN-derived exosomes loaded with rno-miR-29b-3p mimics were added to H9c2 cells and incubated for 24 h, followed by protein isolation and Western blot analysis. Adult mice were given the mouse exosomes transfected with mmu - miR-29b-3p mimics or control miRNA through tail vein injection, and the animals were euthanized 24 h later. Individual hearts were collected for Western blot analysis of proteins.

OSB00017W

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