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- References [2]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-33405 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33405, RRID:AB_2107841
- Product name
- Rabbit Polyclonal GCLM Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references A Genome-wide Haploid Genetic Screen Identifies Regulators of Glutathione Abundance and Ferroptosis Sensitivity.
Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype.
Cao JY, Poddar A, Magtanong L, Lumb JH, Mileur TR, Reid MA, Dovey CM, Wang J, Locasale JW, Stone E, Cole SPC, Carette JE, Dixon SJ
Cell reports 2019 Feb 5;26(6):1544-1556.e8
Cell reports 2019 Feb 5;26(6):1544-1556.e8
Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype.
Lu H, Samanta D, Xiang L, Zhang H, Hu H, Chen I, Bullen JW, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2015 Aug 18;112(33):E4600-9
Proceedings of the National Academy of Sciences of the United States of America 2015 Aug 18;112(33):E4600-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GCLM Antibody [NBP1-33405] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GCLM Antibody [NBP1-33405] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody [N1N3]. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GCLM Antibody [NBP1-33405] - Paraffin-embedded BT474 xenograft, using antibody at 1:500 dilution.
