PA5-29384

antibody from Invitrogen Antibodies

Targeting: GCLM GLCLR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29384

Provider

Invitrogen Antibodies

Product name

GCLM Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: HeLa, 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.

Concentration

1 mg/mL

References

Submitted references

HNF1β drives glutathione (GSH) synthesis underlying intrinsic carboplatin resistance of ovarian clear cell carcinoma (OCCC).

Lopes-Coelho F, Gouveia-Fernandes S, Gonçalves LG, Nunes C, Faustino I, Silva F, Félix A, Pereira SA, Serpa J
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Apr;37(4):4813-29

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using GCLM Polyclonal Antibody (Product # PA5-29384). Sample (30 µg of whole cell lysate). Lane A: H1299. 12% SDS PAGE. GCLM Polyclonal Antibody (Product # PA5-29384) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GCLM in paraformaldehyde-fixed HeLa cells using a GCLM polyclonal antibody (Product # PA5-29384) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using GCLM (Product # PA5-29384) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.