PA5-29908

antibody from Invitrogen Antibodies

Targeting: GCLM GLCLR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29908

Provider

Invitrogen Antibodies

Product name

GCLM Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, A431, mouse liver, rat liver, rat kidney.

Concentration

0.33 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GCLM using 30 µg of C8D30 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a GCLM polyclonal antibody (Product # PA5-29908) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GCLM was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a GCLM Polyclonal Antibody (Product # PA5-29908) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using GCLM Polyclonal Antibody (Product # PA5-29908). Sample (50 µg of whole cell lysate). Lane A: rat liver. 12% SDS PAGE. GCLM Polyclonal Antibody (Product # PA5-29908) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GCLM was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a GCLM Polyclonal Antibody (Product # PA5-29908) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using GCLM Polyclonal Antibody (Product # PA5-29908). Sample (30 µg of whole cell lysate). Lane A: A431. 12% SDS PAGE. GCLM Polyclonal Antibody. GCLM Polyclonal Antibody (Product # PA5-29908) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GCLM in methanol-fixed HeLa cells using a GCLM polyclonal antibody (Product # PA5-29908) at a 1:200 dilution.