HPA036359

antibody from Atlas Antibodies

Targeting: GCLC GCS, GLCL, GLCLC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA036359

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA036359, RRID:AB_10672243

Product name

Anti-GCLC

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

DEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDK
NTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMG
NCCLQVTFQACSISEARYLYDQLAT


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines A-549 and U-251MG using Anti-GCLC antibody. Corresponding GCLC RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line RT-4.

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.

Sample type

HUMAN