R31614

antibody from NSJ Bioreagents

Targeting: GCLC GCS, GLCL, GLCLC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31614

Provider

NSJ Bioreagents

Product name

GCLC Antibody

Antibody type

Polyclonal

Description

This highly specific GCLC antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the GCLC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of GCLC antibody and Lane 1: rat brain; 2: (r) heart; 3: human HeLa; 4: (r) PC12; 5: (r) NRK; 6: mouse HEPA; 7: (h) A549. Predicted/observed size ~72KD

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of GCLC antibody and recombinant human protein (0.5ng)

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) A549, 2) RT4, 3) HepG2, 4) A431, 5) rat liver, 6) rat kidney, 7) mouse liver and 8) mouse kidney tissue lysate with GCLC antibody. Predicted molecular weight ~72 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: GCLC antibody testing of human lung cancer tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: GCLC antibody testing of human breast cancer tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colorectal cancer tissue with GCLC antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human A549 cells with GCLC antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GCLC antibody.