Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- 12601-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GCLC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of paraffin-embedded human liver tissue slide using 12601-1-AP (GCLC antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of paraffin-embedded human liver tissue slide using 12601-1-AP (GCLC antibody) at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 12601-1-AP ( GCLC Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 12601-1-AP ( GCLC Antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-GCLC (IP:12601-1-AP, 3ug; Detection:12601-1-AP 1:500) with mouse kidney tissue lysate 4000ug.