LS-C357544

antibody from LSBio

Targeting: SERPINB5 maspin, PI5

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C357544

Provider

LSBio

Product name

SERPINB5 / Maspin Antibody (aa1-350) LS-C357544

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MASPIN antibody Western blot. All lanes: Anti MASPIN at 0.5 ug/ml. Lane 1: Mouse Intestine Tissue Lysate at 50 ug. Lane 2: Human Placenta Tissue Lysate at 50 ug. Lane 3: MCF-7 Whole Cell Lysate at 40 ug. Lane 4: MM231 Whole Cell Lysate at 40 ug. Predicted band size: 42 kD. Observed band size: 42 kD.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of MASPIN using anti-MASPIN antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: mouse small intestine tissue lysates, Lane 3: human placenta tissue lysates, Lane 4: human MDA-MB-231 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MASPIN antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MASPIN at approximately 42KD. The expected band size for MASPIN is at 42KD.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MASPIN antibody ICC: MCF-7 Cell.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

MASPIN antibody ICC: MCF-7 Cell.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

MASPIN antibody ICC: MCF-7 Cell.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MASPIN antibody IHC-paraffin: Mouse Intestine Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MASPIN antibody IHC-paraffin: Rat Intestine Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MASPIN antibody IHC-paraffin: Human Mammary Cancer Tissue.