R31769

antibody from NSJ Bioreagents

Targeting: SERPINB5 maspin, PI5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31769

Provider

NSJ Bioreagents

Product name

SERPINB5 Antibody / MASPIN

Antibody type

Polyclonal

Description

This highly specific SERPINB5 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the SERPINB5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of Ovalbumin antibody and Lane 1: mouse intestine; 2: human placenta; 3: (h) MCF-7; 4: (h) MM231 lysate. Predicted/observed molecular weight: 42~45kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) HaCaT and 3) Caco-2 cell lysate with SERPINB5 antibody. Predicted molecular weight: 42~45 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ICC testing of Ovalbumin antibody and MCF-7 cells

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human A431 cells with SERPINB5 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: Ovalbumin antibody testing of human breast cancer tissue. HIER: steamed with pH6 citrate buffer.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P testing of mouse intestine tissue. HIER: steamed with pH6 citrate buffer.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P testing of rat intestine tissue. HIER: steamed with pH6 citrate buffer.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colon cancer tissue with SERPINB5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse colon tissue with SERPINB5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.