PA5-27196

antibody from Invitrogen Antibodies

Targeting: SERPINB5 maspin, PI5

Antibody data

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Validation data

SERPINB5 protein structure - PA5-27196 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Maspin Polyclonal Antibody (Product # PA5-27196). Sample (30 µg whole cell lysate). A: HeLa S3. 10% SDS PAGE. Maspin Polyclonal Antibody (Product # PA5-27196) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Maspin Polyclonal Antibody (Product # PA5-27196). Sample (30 µg of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. Maspin Polyclonal Antibody (Product # PA5-27196) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Maspin Polyclonal Antibody(Product # PA5-27196) and a 42kDa band corresponding to Maspin was observed across cell lines and tissue extract tested except Caco-2, MDA-MB-231 and Mouse Adipose which is reported to be negative. Whole cell extracts (30 µg lysate) of MCF 10A (Lane 1), HaCaT (Lane 2), HeLa (Lane 3), Caco-2 (Lane 4), MDA-MB-231 (Lane 5) and tissue extracts of Mouse Adipose (Lane 6) and Mouse Mammary gland (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:3000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).