PA1-16826

antibody from Invitrogen Antibodies

Targeting: CENPF hcp-1

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

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Validation data

CENPF protein structure - PA1-16826 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of CENPF in HeLa cells. Samples were incubated in CENPF polyclonal antibody (Product # PA1-16826) followed by Alexa Fluor 488-conjugated Goat to rabbit IgG secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of CENPF in mitotic spindle. Samples were incubated in CENPF polyclonal antibody (Product # PA1-16826). Distribution of CENP-F (red), largely associated with the mitotic spindle and a clearly distinguishable frac.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of CENPF was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CENPF Polyclonal Antibody (Product # PA1-16826) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034, 1:2000 dilution) for 45 minutes at room temperature (Panel a: green) in HeLa cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of HeLa cells showing nuclear and cytoplasmic localization for CENPF. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.