A301-611A

antibody from Invitrogen Antibodies

Targeting: CENPF hcp-1

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-611A

Provider

Invitrogen Antibodies

Product name

CENP-F/Mitosin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CENP-F/Mitosin by immunocytochemistry. Samples: Acetone-fixed HeLa cells. Antibody: Affinity purified rabbit anti-CENP-F/Mitosin (Cat. No. A301-611A) used at a dilution of 1:1,000 (1 µg/mL). Detection: Anti-rabbit IgG-FITC conjugated (Cat. No. A120-101F) used at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CENP-F/Mitosin by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CENP-F/Mitosin antibody A301-611A used for IP at 3 µg/mg lysate. CENP-F/Mitosin was also immunoprecipitated by rabbit anti-CENP-F/Mitosin antibodies A301-612A and A301-617A, which recognize downstream epitopes. For blotting immunoprecipitated CENP-F/Mitosin, A301-617A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.