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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-20610 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal TFIIE beta Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TFIIE beta Antibody [NBP2-20610] - Sample (30 ug of whole cell lysate) A: Jurkat nucleus 10% SDS PAGE gel, diluted at 1:5000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TFIIE beta Antibody [NBP2-20610] - IP Sample: Jurkat whole cell lysate/extract A : 30 ug whole cell lysate/extract of TFIIE beta protein expressing Jurkat cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of TFIIE beta by 2.5 ug of TFIIE beta antibody 12% SDS-PAGE The immunoprecipitated TFIIE beta protein was detected by TFIIE beta antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: TFIIE beta Antibody [NBP2-20610] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-TFIIE beta antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.
