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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-28177 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GTF2E2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat nucleus. Predicted reactivity: Mouse (95%), Rat (95%), Zebrafish (82%), Xenopus laevis (89%), Pig (96%), Chicken (84%), Rhesus Monkey (99%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GTF2E2 Polyclonal Antibody (Product # PA5-28177). Sample (30 µg of whole cell lysate). Lane A: Jurkat nucleus. 10% SDS PAGE. GTF2E2 Polyclonal Antibody (Product # PA5-28177) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or GTF2E2 Polyclonal Antibody (Product # PA5-28177). The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GTF2E2 Polyclonal Antibody immunoprecipitates TFIIE beta protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A : 30 µg whole cell lysate/extract of TFIIE beta protein expressing Jurkat cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of TFIIE beta by 2.5 µg of GTF2E2 Polyclonal Antibody (Product # PA5-28177) 12% SDS-PAGE The immunoprecipitated TFIIE beta protein was detected by GTF2E2 Polyclonal Antibody (Product # PA5-28177) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
