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LearnMA5-13446
antibody from Invitrogen Antibodies
Targeting: SNRPB
COD, Sm-B/B', SmB/SmB', snRNP-B, SNRPB1
Western blot
Immunohistochemistry
Immunoelectron microscopyAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- MA5-13446 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNRPB Monoclonal Antibody (Y12), Biotin
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Drosophila, Porcine, Rabbit
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- Y12
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references Spliceosome integrity is defective in the motor neuron diseases ALS and SMA.
Mutation of a U2 snRNA gene causes global disruption of alternative splicing and neurodegeneration.
PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa.
The inability of fully grown germinal vesicle stage oocyte cytoplasm to transcriptionally silence transferred transcribing nuclei.
Functional association of the Microprocessor complex with the spliceosome.
Ribonucleoprotein assembly defects correlate with spinal muscular atrophy severity and preferentially affect a subset of spliceosomal snRNPs.
Conserved patterns of nuclear compartmentalization are not observed in the chordate Oikopleura.
Inhibition of U snRNP assembly by a virus-encoded proteinase.
Mutations in splicing factor PRPF3, causing retinal degeneration, form detrimental aggregates in photoreceptor cells.
Myosin16b: The COOH-tail region directs localization to the nucleus and overexpression delays S-phase progression.
Arginine methyltransferase Capsuleen is essential for methylation of spliceosomal Sm proteins and germ cell formation in Drosophila.
Restoration of SMN function: delivery of a trans-splicing RNA re-directs SMN2 pre-mRNA splicing.
Gemin8 is a novel component of the survival motor neuron complex and functions in small nuclear ribonucleoprotein assembly.
Nuclear substructure reorganization during late-stage erythropoiesis is selective and does not involve caspase cleavage of major nuclear substructural proteins.
Subcellular localization of protein kinase C delta and epsilon affects transcriptional and post-transcriptional processes in four-cell mouse embryos.
Tim50a, a nuclear isoform of the mitochondrial Tim50, interacts with proteins involved in snRNP biogenesis.
A key role for stress-induced satellite III transcripts in the relocalization of splicing factors into nuclear stress granules.
Coupled in vitro import of U snRNPs and SMN, the spinal muscular atrophy protein.
ELL and EAF1 are Cajal body components that are disrupted in MLL-ELL leukemia.
pICln inhibits snRNP biogenesis by binding core spliceosomal proteins.
pICln inhibits snRNP biogenesis by binding core spliceosomal proteins.
Tsuiji H, Iguchi Y, Furuya A, Kataoka A, Hatsuta H, Atsuta N, Tanaka F, Hashizume Y, Akatsu H, Murayama S, Sobue G, Yamanaka K
EMBO molecular medicine 2013 Feb;5(2):221-34
EMBO molecular medicine 2013 Feb;5(2):221-34
Mutation of a U2 snRNA gene causes global disruption of alternative splicing and neurodegeneration.
Jia Y, Mu JC, Ackerman SL
Cell 2012 Jan 20;148(1-2):296-308
Cell 2012 Jan 20;148(1-2):296-308
PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa.
Tanackovic G, Ransijn A, Thibault P, Abou Elela S, Klinck R, Berson EL, Chabot B, Rivolta C
Human molecular genetics 2011 Jun 1;20(11):2116-30
Human molecular genetics 2011 Jun 1;20(11):2116-30
The inability of fully grown germinal vesicle stage oocyte cytoplasm to transcriptionally silence transferred transcribing nuclei.
Fulka H, Novakova Z, Mosko T, Fulka J Jr
Histochemistry and cell biology 2009 Oct;132(4):457-68
Histochemistry and cell biology 2009 Oct;132(4):457-68
Functional association of the Microprocessor complex with the spliceosome.
Kataoka N, Fujita M, Ohno M
Molecular and cellular biology 2009 Jun;29(12):3243-54
Molecular and cellular biology 2009 Jun;29(12):3243-54
Ribonucleoprotein assembly defects correlate with spinal muscular atrophy severity and preferentially affect a subset of spliceosomal snRNPs.
Gabanella F, Butchbach ME, Saieva L, Carissimi C, Burghes AH, Pellizzoni L
PloS one 2007 Sep 26;2(9):e921
PloS one 2007 Sep 26;2(9):e921
Conserved patterns of nuclear compartmentalization are not observed in the chordate Oikopleura.
Spada F, Koch J, Sadoni N, Mitchell N, Ganot P, De Boni U, Zink D, Thompson EM
Biology of the cell 2007 May;99(5):273-87
Biology of the cell 2007 May;99(5):273-87
Inhibition of U snRNP assembly by a virus-encoded proteinase.
Almstead LL, Sarnow P
Genes & development 2007 May 1;21(9):1086-97
Genes & development 2007 May 1;21(9):1086-97
Mutations in splicing factor PRPF3, causing retinal degeneration, form detrimental aggregates in photoreceptor cells.
Comitato A, Spampanato C, Chakarova C, Sanges D, Bhattacharya SS, Marigo V
Human molecular genetics 2007 Jul 15;16(14):1699-707
Human molecular genetics 2007 Jul 15;16(14):1699-707
Myosin16b: The COOH-tail region directs localization to the nucleus and overexpression delays S-phase progression.
Cameron RS, Liu C, Mixon AS, Pihkala JP, Rahn RJ, Cameron PL
Cell motility and the cytoskeleton 2007 Jan;64(1):19-48
Cell motility and the cytoskeleton 2007 Jan;64(1):19-48
Arginine methyltransferase Capsuleen is essential for methylation of spliceosomal Sm proteins and germ cell formation in Drosophila.
Anne J, Ollo R, Ephrussi A, Mechler BM
Development (Cambridge, England) 2007 Jan;134(1):137-46
Development (Cambridge, England) 2007 Jan;134(1):137-46
Restoration of SMN function: delivery of a trans-splicing RNA re-directs SMN2 pre-mRNA splicing.
Coady TH, Shababi M, Tullis GE, Lorson CL
Molecular therapy : the journal of the American Society of Gene Therapy 2007 Aug;15(8):1471-8
Molecular therapy : the journal of the American Society of Gene Therapy 2007 Aug;15(8):1471-8
Gemin8 is a novel component of the survival motor neuron complex and functions in small nuclear ribonucleoprotein assembly.
Carissimi C, Saieva L, Baccon J, Chiarella P, Maiolica A, Sawyer A, Rappsilber J, Pellizzoni L
The Journal of biological chemistry 2006 Mar 24;281(12):8126-34
The Journal of biological chemistry 2006 Mar 24;281(12):8126-34
Nuclear substructure reorganization during late-stage erythropoiesis is selective and does not involve caspase cleavage of major nuclear substructural proteins.
Krauss SW, Lo AJ, Short SA, Koury MJ, Mohandas N, Chasis JA
Blood 2005 Sep 15;106(6):2200-5
Blood 2005 Sep 15;106(6):2200-5
Subcellular localization of protein kinase C delta and epsilon affects transcriptional and post-transcriptional processes in four-cell mouse embryos.
Dehghani H, Reith C, Hahnel AC
Reproduction (Cambridge, England) 2005 Oct;130(4):453-65
Reproduction (Cambridge, England) 2005 Oct;130(4):453-65
Tim50a, a nuclear isoform of the mitochondrial Tim50, interacts with proteins involved in snRNP biogenesis.
Xu H, Somers ZB, Robinson ML 2nd, Hebert MD
BMC cell biology 2005 Jul 11;6(1):29
BMC cell biology 2005 Jul 11;6(1):29
A key role for stress-induced satellite III transcripts in the relocalization of splicing factors into nuclear stress granules.
Metz A, Soret J, Vourc'h C, Tazi J, Jolly C
Journal of cell science 2004 Sep 1;117(Pt 19):4551-8
Journal of cell science 2004 Sep 1;117(Pt 19):4551-8
Coupled in vitro import of U snRNPs and SMN, the spinal muscular atrophy protein.
Narayanan U, Achsel T, Lührmann R, Matera AG
Molecular cell 2004 Oct 22;16(2):223-34
Molecular cell 2004 Oct 22;16(2):223-34
ELL and EAF1 are Cajal body components that are disrupted in MLL-ELL leukemia.
Polak PE, Simone F, Kaberlein JJ, Luo RT, Thirman MJ
Molecular biology of the cell 2003 Apr;14(4):1517-28
Molecular biology of the cell 2003 Apr;14(4):1517-28
pICln inhibits snRNP biogenesis by binding core spliceosomal proteins.
Pu WT, Krapivinsky GB, Krapivinsky L, Clapham DE
Molecular and cellular biology 1999 Jun;19(6):4113-20
Molecular and cellular biology 1999 Jun;19(6):4113-20
pICln inhibits snRNP biogenesis by binding core spliceosomal proteins.
Pu WT, Krapivinsky GB, Krapivinsky L, Clapham DE
Molecular and cellular biology 1999 Jun;19(6):4113-20
Molecular and cellular biology 1999 Jun;19(6):4113-20
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), NTERA-2 (Lane 2), NIH/3T3 (Lane 3), Hep G2 (Lane 4), K-562 (Lane 5), SH-SY5Y (Lane 6), HEK-293 (Lane 7) and A-431 (Lane 8). The blots were probed with Anti-SNRPB Monoclonal Antibody (Product # MA5-13446, 1 µg/mL) and detected by chemiluminescence. A 24 kDa and 30 kDa band corresponding to Smith antigen was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary antibody following blocking with 5 % skimmed milk. This is followed by incubating the membrane with Poly-HRP Streptavidin (Product # N200, 1:10,000). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SNRPB was achieved by transfecting HeLa cells with SNRPB specific siRNAs (Silencer® select Product # s13220 and s13221). Western blot analysis (Fig a) was performed using Modified whole cell extract from the SNRPB knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-SNRPB biotin Mouse Monoclonal Antibody (Product # MA5-13449, 0.2 µg/mL) and Poly-HRP Streptavidin (Product # N200, 1:10,000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to SNRPB.
- Conjugate
- Biotin
