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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [4]
- Immunohistochemistry [2]
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- Product number
- LS-C313514 - Provider product page
- Provider
- LSBio
- Product name
- SNK / PLK2 Antibody (aa666-685) LS-C313514
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Rat, Guinea Pig, Horse
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- WB of SNK / PLK2 antibody. Lane 1: A431 Cell Lysate. Lane 2: 293T Cell Lysate. Lane 3: COLO320 Cell Lysate.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SNK / PLK2 antibody. IHC(P); Rat Brain Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ICC analysis of PLK2 using anti-PLK2 antibody. PLK2 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-PLK2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- SNK / PLK2 antibody. IHC(P); Rat Brain Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ICC analysis of PLK2 using anti-PLK2 antibody. PLK2 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-PLK2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SNK / PLK2 antibody. IHC(P): Human Lung Cancer Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SNK / PLK2 antibody. IHC(P): Rat Intestine Tissue.
