628906

antibody from BioLegend

Targeting: PLK2 SNK

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

628906

Provider

BioLegend

Product name

Alexa Fluor® 488 anti-PLK-1 Phospho (Thr210) [2A3]

Antibody type

Monoclonal

Antigen

Modified peptide

Description

PLK-1 (polo-like kinase 1) is a member of te serine/threonine protein kinase family, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division; depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The 2A3 antibody recognizes human phosphorylated PLK-1 (Thr210) and has been shown to be useful for Western blotting. To increase specificity, it is recommended that the 2A3 antibody be used for Western blotting after immunoprecipitation with the pan-specific PLK-1 3F8 antibody.

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Vial size

100 tests

Storage

The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.