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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- MA5-17231 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CK1 epsilon Monoclonal Antibody (AF6C1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- A suggested positive control for this product is 293T cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AF6C1
- Vial size
- 100 µL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Casein kinase 1 e using anti-Casein kinase 1 e monoclonal antibody (Product # MA5-17231) on (Lane 1) HeLa Cell lysate, (Lane 2) Ramos Cell lysate, (Lane 3) A431 Cell lysate, (Lane 4) 293T Cell lysate, (Lane 5) Jurket Cell lysate and (Lane 6) PC12 Cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of CK1 epsilon was achieved by transfecting U-87 MG with CK1 epsilon specific siRNAs (Silencer® select Product # s57). Western blot analysis (Fig. a) was performed using whole cell extracts from the CK1 epsilon knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with CK1 epsilon Monoclonal Antibody (AF6C1) (Product # MA5-17231, 1:2000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CK1 epsilon.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CK1 epsilon Monoclonal Antibody (AF6C1) (Product # MA5-17231) and a 47 kDa band corresponding to CK1 epsilon was observed across all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), HeLa (Lane 2), MDA-MB-231 (Lane 3), HEK-293 (Lane 4), Jurkat (Lane 5), NIH/3T3 (Lane 6), tissue extracts of Mouse Brain (Lane 7) and Rat Brain (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
