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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB100-93388 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-93388, RRID:AB_1236830
- Product name
- Goat Polyclonal Casein Kinase 1 epsilon Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Both reported variants represent identical protein (NP_001885.1 and NP_689407.1).
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-PASQTSVPFDHLGK- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references A second protein kinase CK1-mediated step negatively regulates Wnt signalling by disrupting the lymphocyte enhancer factor-1/beta-catenin complex.
Hämmerlein A, Weiske J, Huber O
Cellular and molecular life sciences : CMLS 2005 Mar;62(5):606-18
Cellular and molecular life sciences : CMLS 2005 Mar;62(5):606-18
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Casein Kinase 1 epsilon Antibody [NB100-93388] - Staining of HEK293 cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Casein Kinase 1 epsilon Antibody [NB100-93388] - (1ug/ml) staining of A431(A), HeLa (B) and K562 (C) nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Casein Kinase 1 epsilon Antibody [NB100-93388] - Staining of paraffin embedded human tonsil tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Casein Kinase 1 epsilon Antibody [NB100-93388] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
