MA5-26623

antibody from Invitrogen Antibodies

Targeting: CSNK1E CKIE, CKIepsilon, HCKIE

Antibody data

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Validation data

CSNK1E protein structure - MA5-26623 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CSNK1E in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with CSNK1E (Product # MA5-26623) monoclonal antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CSNK1E in HeLa wild-type and knockout cells using 10 µg per lane. Samples were separated by SDS-PAGE and probed with CSNK1E (Product # MA5-26623) monoclonal antibody and HSP90AB1 antibody with a dilution of 1:500 as a loading control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of CK1 epsilon was achieved by transfecting U-87 MG with CK1 epsilon specific siRNAs (Silencer® select Product # s57). Western blot analysis (Fig. a) was performed using whole cell extracts from the CK1 epsilon knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with CK1 epsilon Monoclonal Antibody (OTI2B3) (Product # MA5-26623, 1:2000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CK1 epsilon.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-CK1 epsilon Monoclonal Antibody (OTI2B3) (Product # MA5-26623) and a 47 kDa band corresponding to CK1 epsilon was observed across all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), HeLa (Lane 2), MDA-MB-231 (Lane 3), HEK-293 (Lane 4), Jurkat (Lane 5), NIH/3T3 (Lane 6), PC-12 (Lane 7), tissue extracts of Mouse Brain (Lane 8) and Rat Brain (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).