PA5-95934

antibody from Invitrogen Antibodies

Targeting: CSNK1E CKIE, CKIepsilon, HCKIE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-95934

Provider

Invitrogen Antibodies

Product name

CK1 epsilon Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: EFSTYLNFCR SLRFDDKPDY SYLRQLFRNL FHRQGFSYDY VFDWNMLKFG AARNPEDVDR ERREHEREER MGQLRGSATR ALPPGPPTGA TANRLRSAAE PVASTPASRI QPAGNTSPRA ISRVDRERKV SMRLHRGAPA NVSSSDLTGR QEVSRIPASQ TSVPFDHLGK; Positive Samples: HeLa, 293T; Cellular Location: Cytoplasm, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.93 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CK1 epsilon in extracts from normal (control) and CSNK1E knockout (KO) HeLa cells using CK1 epsilon Polyclonal Antibody (Product # PA5-95934) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CK1 epsilon in extracts of various cell lines using CK1 epsilon Polyclonal Antibody (Product # PA5-95934) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of CK1 epsilon was performed in HeLa cells using CK1 epsilon Polyclonal Antibody (Product # PA5-95934).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CK1 epsilon in paraffin-embedded rat pancreas using CK1 epsilon Polyclonal Antibody (Product # PA5-95934) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CK1 epsilon in paraffin-embedded mouse brain using CK1 epsilon Polyclonal Antibody (Product # PA5-95934) at a dilution of 1:200.