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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- A302-135A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CKI epsilon Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CKI epsilon by western blot. Samples: Whole cell lysate (5, 15, and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-CKI epsilon antibody (Product # A302-135A; lot A302-135A-1) used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CKI epsilon by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CKI epsilon antibody A302-135A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. CKI epsilon was also immunoprecipitated by a rabbit antibody (BL7891) directed to an epitope that is common to CKI epsilon and CKI delta. CKI delta was immunoprecipitated and blotted using A302-136A. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CKI epsilon by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CKI epsilon antibody (Product # A302-135A; lot A302-0135A-1) used for IP at 10 µg/mg lysate. CKI epsilon was also immunoprecipitated by a rabbit antibody (BL7891) directed to an epitope that is common to CKI epsilon and CKI deltA. CKI delta was immunoprecipitated and blotted using Product # A302-136A. For blotting immunoprecipitated CKI epsilon, Product # A302-135A was used at 1.0 µg/mL. Detection: Chemiluminescence with exposure time of 3 seconds.
