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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AM00025PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM00025PU-N, RRID:AB_973786
- Product name
- anti Casein kinase I epsilon (incl. pos. control)
- Antibody type
- Monoclonal
- Antigen
- Peptide (aa 355 - 380 of CK1e) conjugated to hemocyanin
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4D7
- Vial size
- 0.1 mg
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Detection of endogenous Casein Kinase 1 epsilon: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab CK1e-4D7(0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec) lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A; lane 9: NIH 3T3.
