LS-C98724

antibody from LSBio

Targeting: MYLK MLCK, MLCK1, MYLK1, smMLCK

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C98724

Provider

LSBio

Proper citation

LifeSpan Cat#LS-C98724, RRID:AB_2251015

Product name

MYLK Antibody (aa923-953) LS-C98724

Antibody type

Polyclonal

Description

Ammonium sulfate precipitation

Reactivity

Human, Mouse

Host

Rabbit

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of lysate from mouse bladder tissue lysate, using MLCKlong Antibody (M1). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MLCK Antibody (M1) western blot of SK-BR-3 cell line lysates (35 ug/lane). The MLCK antibody detected the MLCK protein (arrow).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of anti-MLCK-long antibody in mouse brain tissue lysate. MLCK-long (Arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.