21642-1-AP

antibody from Proteintech Group

Targeting: MYLK MLCK, MLCK1, MYLK1, smMLCK

Provider product page for 21642-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [22]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 21642-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#21642-1-AP, RRID:AB_10734590
Product name: MYLK antibody
Antibody type: Polyclonal
Description: MYLK antibody (Cat. #21642-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MYLK protein structure - 21642-1-AP shown in red.

Submitted references Trifluoperazine regulates blood-brain barrier permeability via the MLCK/p-MLC pathway to promote ischemic stroke recovery.

Zhang W, Chen S, Ma B, Ding Y, Liu X, He C, Wang B, Yuan M
iScience 2024 Mar 15;27(3):109156

Banxia Xiexin decoction promotes gastric lymphatic pumping by regulating lymphatic smooth muscle cell contraction and energy metabolism in a stress-induced gastric ulceration rat model.

Pan S, Yu X, Liu M, Liu J, Wang C, Zhang Y, Ge F, Fan A, Zhang D, Chen M
Journal of ethnopharmacology 2024 Jun 28;328:118015

An Inulin-Type Fructan CP-A from Codonopsis pilosula Alleviated 5-Fluorouracil-Induced Intestinal Mucositis via the ERK/MLCK/MLC2 Pathway and Regulation of Gut Microbiota.

Zhou J, Li D, Wang J, Cheng Z, Wang C, Zhang X, Xu X, Gao J
Pharmaceuticals (Basel, Switzerland) 2024 Feb 26;17(3)

A synergistic regulation works in matrix stiffness-driven invadopodia formation in HCC.

Zhang X, Zhao Y, Li M, Wang M, Qian J, Wang Z, Wang Y, Wang F, Guo K, Gao D, Zhao Y, Chen R, Ren Z, Song H, Cui J
Cancer letters 2024 Feb 1;582:216597

The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling.

Chen X, Xu Z, Tang K, Hu G, Du P, Wang J, Zhang C, Xin Y, Li K, Zhang Q, Hu J, Zhang Z, Yang M, Wang G, Tan Y
Research (Washington, D.C.) 2023;6:0224

Single-cell RNA sequencing reveals tumor heterogeneity within salivary gland pleomorphic adenoma: A preliminary study.

Wang XQ, Zhong NN, Man QW, Xu GC, Yan SC, Peng LW, Wang YG, Liu B, Bu LL, Li L
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2023 Sep;52(8):766-776

GPR65 inhibits human trophoblast cell adhesion through upregulation of MYLK and downregulation of fibronectin via cAMP-ERK signaling in a low pH environment.

Mao J, Feng Y, Zheng Y, Gao Y, Zhang L, Sun X, Wu Y, Zhu X, Ma F
Cell communication and signaling : CCS 2023 Sep 18;21(1):238

Chlorogenic Acid Ameliorates Post-Infectious Irritable Bowel Syndrome by Regulating Extracellular Vesicles of Gut Microbes.

Zheng C, Zhong Y, Zhang W, Wang Z, Xiao H, Zhang W, Xie J, Peng X, Luo J, Xu W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Oct;10(28):e2302798

Inhibition of UTX/KDM6A improves recovery of spinal cord injury by attenuating BSCB permeability and macrophage infiltration through the MLCK/p-MLC pathway.

Xie Y, Luo Z, Peng W, Liu Y, Yuan F, Xu J, Sun Y, Lu H, Wu T, Jiang L, Hu J
Journal of neuroinflammation 2023 Nov 11;20(1):259

Tea Polyphenols Protects Tracheal Epithelial Tight Junctions in Lung during Actinobacillus pleuropneumoniae Infection via Suppressing TLR-4/MAPK/PKC-MLCK Signaling.

Li X, Liu Z, Gao T, Liu W, Yang K, Guo R, Li C, Tian Y, Wang N, Zhou D, Bei W, Yuan F
International journal of molecular sciences 2023 Jul 24;24(14)

Morphological and Functional Colonic Defects Caused by a Mutated Thyroid Hormone Receptor α.

Kim M, Kruhlak M, Hoffmann V, Zerfas P, Bishop K, Doolittle WKL, Edmondson EF, Zhu YJ, Cheng SY
Thyroid : official journal of the American Thyroid Association 2023 Feb;33(2):239-250

Vaccinia virus induces EMT-like transformation and RhoA-mediated mesenchymal migration.

Liu W, Lu JY, Wang YJ, Xu XX, Chen YC, Yu SX, Xiang XW, Chen XZ, Jiu Y, Gao H, Sheng M, Chen ZJ, Hu X, Li D, Maiuri P, Huang X, Ying T, Xu GL, Pang DW, Zhang ZL, Liu B, Liu YJ
Journal of medical virology 2023 Aug;95(8):e29041

Heat-Killed Lacticaseibacillus paracasei Repairs Lipopolysaccharide-Induced Intestinal Epithelial Barrier Damage via MLCK/MLC Pathway Activation.

Xie Z, Zhang G, Liu R, Wang Y, Tsapieva AN, Zhang L, Han J
Nutrients 2023 Apr 4;15(7)

Intestinal Flora-Derived Kynurenic Acid Protects Against Intestinal Damage Caused by Candida albicans Infection via Activation of Aryl Hydrocarbon Receptor.

Wang Z, Yin L, Qi Y, Zhang J, Zhu H, Tang J
Frontiers in microbiology 2022;13:934786

The m(6)A methyltransferase METTL3 promotes trophoblast cell invasion by regulating MYLK expression.

Zhao J, Ding H, Ding J, Shi X, He Y, Zhu H, Yuan H, Zhang T, Zhang J
Placenta 2022 Nov;129:1-6

Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.

He F, Ding H, Zhou Y, Wang Y, Xie J, Yang S, Zhu Y
Frontiers in medicine 2021;8:792740

Protective effects of curcumin against rat intestinal inflammation‑related motility disorders.

Yao Y, Luo R, Xiong S, Zhang C, Zhang Y
Molecular medicine reports 2021 May;23(5)

Lanthanum chloride causes blood-brain barrier disruption through intracellular calcium-mediated RhoA/Rho kinase signaling and myosin light chain kinase.

Wu J, Yang J, Yu M, Sun W, Han Y, Lu X, Jin C, Wu S, Cai Y
Metallomics : integrated biometal science 2020 Dec 23;12(12):2075-2083

Doxorubicin induces detrusor smooth muscle impairments through myosin dysregulation, leading to a risk of lower urinary tract dysfunction.

Iguchi N, Dönmez Mİ, Carrasco A Jr, Wilcox DT, Pineda RH, Malykhina AP, Cost NG
American journal of physiology. Renal physiology 2019 Jul 1;317(1):F197-F206

Aryl Hydrocarbon Receptor Activation Modulates Intestinal Epithelial Barrier Function by Maintaining Tight Junction Integrity.

Yu M, Wang Q, Ma Y, Li L, Yu K, Zhang Z, Chen G, Li X, Xiao W, Xu P, Yang H
International journal of biological sciences 2018;14(1):69-77

Dihydroartemisinin counteracts fibrotic portal hypertension via farnesoid X receptor-dependent inhibition of hepatic stellate cell contraction.

Xu W, Lu C, Zhang F, Shao J, Yao S, Zheng S
The FEBS journal 2017 Jan;284(1):114-133

Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment.

Sumagin R, Robin AZ, Nusrat A, Parkos CA
Mucosal immunology 2014 Jul;7(4):905-15

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse uterus tissue were subjected to SDS PAGE followed by western blot with 21642-1-AP(MYLK antibody) at dilution of 1:500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using MYLK antibody 21642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line