21642-1-AP

antibody from Proteintech Group

Targeting: MYLK MLCK, MLCK1, MYLK1, smMLCK

Provider product page for 21642-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [50]
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Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 21642-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#21642-1-AP, RRID:AB_10734590
Product name: MYLK antibody
Antibody type: Polyclonal
Description: MYLK antibody (Cat. #21642-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MYLK protein structure - 21642-1-AP shown in red.

Submitted references Phillyrin improves pulmonary epithelial barrier dysfunction in LPS-induced acute lung injury through the RhoA/ROCK signaling pathway.

Wang Y, Wu Q, Li L, Ke Y, Zhuang Y, Cheng B, Huang M, Wang Y
The Journal of pharmacy and pharmacology 2026 Mar 5;78(3)

Distinct signaling mechanisms and proteome phenotypes are elicited by compartment-specific genetic defects of copper homeostasis.

Lane AR, Gonzalez N, Gokhale A, Zlatic SA, Allen BM, Scher NE, Roberts AM, Duong DM, Roberts BR, Vrailas-Mortimer AD, Werner E, Faundez V
bioRxiv : the preprint server for biology 2026 Jan 14;

Short-chain fatty acids alleviated fluoride-induced neuroinflammation via the gut-brain axis in rats.

Zhao WP, Zhu SQ, Yin ZH, Ommati MM, Zhang YW, Zhao J, Zhou BH, Wang HW
Environmental pollution (Barking, Essex : 1987) 2026 Feb 1;390:127503

Effect of four traditional Chinese medicine monomers on mechanical barrier damage and inflammation response of IPEC-J2 cells caused by soybean 7S globulin.

Deng Y, Wu X, Wan Y, Deng J, Deng H
Frontiers in veterinary science 2025;12:1548866

eIF3f promotes tumour malignancy by remodelling fatty acid biosynthesis in hepatocellular carcinoma.

Zhou S, Zhang L, You Y, Yu K, Tie X, Gao Y, Chen Y, Yao F, Zhang R, Hao X, Fang C, Li X, Li Q, Wang X
Journal of hepatology 2025 Sep;83(3):712-728

Single-cell transcriptomics reveals cellular evolution underlying pleomorphic adenoma recurrence and malignant transformation.

Li Y, Li Z, Zhao G, Han Q, Ding Z, Wang C, Li C
Scientific reports 2025 Oct 14;15(1):35870

Dual-mechanism mucoadhesive hydrogel integrating pH-independent barriers and autophagy-driven immunotherapy for the treatment of ulcerative colitis.

Wang J, Ouyang S, Lin G, Li D, Ding B, Chen Y, Tong B, Ouyang T, Xu H, Wu W
Acta biomaterialia 2025 Oct 1;205:302-318

Antibacterial hyaluronic acid hydrogel with sustained release of larazotide as effective colitis treatment.

Yu F, Chen Y, Ouyang S, Tong B, Jiang Z, Wang J, Ding B, Mao K, Wu W, Xu H
Journal of controlled release : official journal of the Controlled Release Society 2025 Nov 10;387:114205

Folate Attenuates Ulcerative Colitis via PI3K/AKT/NF-κB/MLCK Axis Inhibition to Restore Intestinal Barrier Integrity.

Zhang S, Cheng T, Chen Y, Wang M, Wang G, Aa J
Biology 2025 Nov 10;14(11)

Proline rich-39 (PR-39) antimicrobial protein alleviated lipopolysaccharide-induced intestinal barrier dysfunction in piglets by altering intestinal flora associated bile acid metabolism and in turn regulating TGR-5/NF-κB/MLCK/MLC pathway.

Liu Z, Qin X, Zhang B, Nong K, Chen W, Yang Z, Lang W, Liu X, Li L, Wang X, Shi H, Zhang H
International journal of biological macromolecules 2025 May;307(Pt 1):141930

The Expression and Molecular Roles of MAMDC2 in MSS Colorectal Cancer with a High Tumor Stromal Ratio.

Liu Y, Qian S, Wei J, He J, Li M, Gao X, Cai H, Wang Y, Han Y, Tan T, Yang M
Biomedicines 2025 May 17;13(5)

Elevated SPARC Disrupts the Intestinal Barrier Integrity in Crohn's Disease by Interacting with OTUD4 and Activating the MYD88/NF-κB Pathway.

Wang J, He Y, Zhu X, Zhu J, Deng Z, Zhang H, Chen Y, Zhang G, Shi T, Chen W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Mar;12(11):e2409419

β-Sitosterol Ameliorates Ulcerative Colitis Through Modulation of the AMPK/MLCK Anti-Inflammatory Pathway.

Zhang Y, Jin X, Xia H, Wu X, Chen W, Zhuang M, Tang S
Journal of biochemical and molecular toxicology 2025 Jun;39(6):e70287

Senescent macrophages induce ferroptosis in skeletal muscle and accelerate osteoarthritis-related muscle atrophy.

Xiang W, Zhang T, Li B, Li S, Zhang B, Fang S, Chen L, Gong Y, Huang B, Feng D, Wu J, Yuan J, Wu Y, Yan X, Jin R, Zhang X, Fang X, Lian J, Chen L, Zhou S, Ni Z
Nature aging 2025 Jul;5(7):1295-1316

FoxO4 reduces the damage and mechanism of PEDV-infected IPEC-J2 cells through the NF-κB/MLCK pathway.

Wei Y, Zhao S, Zhao H, Bao D, Liu J, Shao H, Zhang S, Yi S, Wang K, Hu G
Veterinary microbiology 2025 Jul;306:110568

Qingshu Yiqi decoction ameliorates exertional heat stroke-induced intestinal barrier injury via NF-κB/MLC pathway and gut microbiota.

Ma W, Wang M, Chen J, Wang Y, Chen J, Pei Y, Gong Y, You J, Cao Y, Zhou J, Ma J, Chang W, Zhu H, Song Q, Li L, Xu S, Zhu H
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Jul 25;143:156723

Construction of a prostate adenocarcinoma molecular classification: integrating spatial transcriptomics with retrospective cohort validation.

Lu B, Liu Y, Ji G, Yao Y, Yang Z, Zhu B, Wang L, Dong K, Li Y, Shi J, He J, Huang R, Zhou W, Cui X, Pan X, Cui X
Journal of translational medicine 2025 Jul 1;23(1):717

Inulin alleviates intestinal barrier dysfunction induced by chronic intermittent hypoxia by modulating intestinal microbiota in mice.

Xue Y, Tang R, Liu Z
American journal of physiology. Regulatory, integrative and comparative physiology 2025 Dec 1;329(6):R859-R871

MLCK inhibition induces synthetic lethality in MYC-driven cancer.

Sun Z, Wu R, Liang X, Shi T, Zhang Y, Pan Z, Zhang W, Luan X
Cancer letters 2025 Aug 10;625:217803

Androgen deficiency-induced loss of Lactobacillus salivarius extracellular vesicles is associated with the pathogenesis of osteoporosis.

Zhang W, Xie J, Wang Z, Zhong Y, Liu L, Liu J, Zhang W, Pi Y, Tang F, Liu Z, Shao Y, Liu T, Zheng C, Luo J
Microbiological research 2025 Apr;293:128047

Chronic Stress-induced Serotonin Impairs Intestinal Epithelial Cell Mitochondrial Biogenesis via the AMPK-PGC-1α Axis.

Yang D, Sun Y, Wen P, Chen Y, Cao J, Sun X, Dong Y
International journal of biological sciences 2024;20(11):4476-4495

Butyric acid alleviates LPS-induced intestinal mucosal barrier damage by inhibiting the RhoA/ROCK2/MLCK signaling pathway in Caco2 cells.

Liu L, Chen T, Xie Z, Zhang Y, He C, Huang Y
PloS one 2024;19(12):e0316362

Resolvin E1 and Inhibition of BLT2 Signaling Attenuate the Inflammatory Response and Improve One-Lung Ventilation-Induced Lung Injury.

Ji L, Liu G, Yu G, Xia C, Liu S, Lan Y
Immunological investigations 2024 Nov;53(8):1293-1307

A transient blood IL-17 increase triggers neuroinflammation in cerebellum and motor incoordination in hyperammonemic rats.

Arenas YM, Montoliu C, Llansola M, Felipo V
Journal of neuroinflammation 2024 Nov 30;21(1):314

Effects of Saccharomyces boulardii on microbiota composition and metabolite levels in the small intestine of constipated mice.

Tang S, Li J, Li Y, Du H, Zhu W, Zhang R, Wan J
BMC microbiology 2024 Nov 23;24(1):493

The SNHG12/microRNA-15b-5p/MYLK axis regulates vascular smooth muscle cell phenotype to affect intracranial aneurysm formation.

Feng W, Liang H, Liu D, Ruan S
Microvascular research 2024 Mar;152:104643

Trifluoperazine regulates blood-brain barrier permeability via the MLCK/p-MLC pathway to promote ischemic stroke recovery.

Zhang W, Chen S, Ma B, Ding Y, Liu X, He C, Wang B, Yuan M
iScience 2024 Mar 15;27(3):109156

Banxia Xiexin decoction promotes gastric lymphatic pumping by regulating lymphatic smooth muscle cell contraction and energy metabolism in a stress-induced gastric ulceration rat model.

Pan S, Yu X, Liu M, Liu J, Wang C, Zhang Y, Ge F, Fan A, Zhang D, Chen M
Journal of ethnopharmacology 2024 Jun 28;328:118015

An Inulin-Type Fructan CP-A from Codonopsis pilosula Alleviated 5-Fluorouracil-Induced Intestinal Mucositis via the ERK/MLCK/MLC2 Pathway and Regulation of Gut Microbiota.

Zhou J, Li D, Wang J, Cheng Z, Wang C, Zhang X, Xu X, Gao J
Pharmaceuticals (Basel, Switzerland) 2024 Feb 26;17(3)

A synergistic regulation works in matrix stiffness-driven invadopodia formation in HCC.

Zhang X, Zhao Y, Li M, Wang M, Qian J, Wang Z, Wang Y, Wang F, Guo K, Gao D, Zhao Y, Chen R, Ren Z, Song H, Cui J
Cancer letters 2024 Feb 1;582:216597

Therapeutic potential of Lacticaseibacillus rhamnosus grx10 and its derived postbiotic through gut microbiota and MAPK/MLCK/MLC pathway-mediated intestinal barrier repairment in ulcerative colitis.

Ye H, Liu X, Guan K, Ma Y, Liu R, Liu Y, Lv X, Wang R, Li Q
Journal of food science 2024 Dec;89(12):10035-10052

AHR activation relieves deoxynivalenol-induced disruption of porcine intestinal epithelial barrier functions.

Hu ZY, Yang SJ, Chang YH, Wang XQ, Liu RQ, Jiang FW, Chen MS, Wang JX, Liu S, Zhu HM, Shi YS, Zhao Y, Li JL
Journal of hazardous materials 2024 Dec 5;480:136095

The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling.

Chen X, Xu Z, Tang K, Hu G, Du P, Wang J, Zhang C, Xin Y, Li K, Zhang Q, Hu J, Zhang Z, Yang M, Wang G, Tan Y
Research (Washington, D.C.) 2023;6:0224

Single-cell RNA sequencing reveals tumor heterogeneity within salivary gland pleomorphic adenoma: A preliminary study.

Wang XQ, Zhong NN, Man QW, Xu GC, Yan SC, Peng LW, Wang YG, Liu B, Bu LL, Li L
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2023 Sep;52(8):766-776

GPR65 inhibits human trophoblast cell adhesion through upregulation of MYLK and downregulation of fibronectin via cAMP-ERK signaling in a low pH environment.

Mao J, Feng Y, Zheng Y, Gao Y, Zhang L, Sun X, Wu Y, Zhu X, Ma F
Cell communication and signaling : CCS 2023 Sep 18;21(1):238

Chlorogenic Acid Ameliorates Post-Infectious Irritable Bowel Syndrome by Regulating Extracellular Vesicles of Gut Microbes.

Zheng C, Zhong Y, Zhang W, Wang Z, Xiao H, Zhang W, Xie J, Peng X, Luo J, Xu W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Oct;10(28):e2302798

Inhibition of UTX/KDM6A improves recovery of spinal cord injury by attenuating BSCB permeability and macrophage infiltration through the MLCK/p-MLC pathway.

Xie Y, Luo Z, Peng W, Liu Y, Yuan F, Xu J, Sun Y, Lu H, Wu T, Jiang L, Hu J
Journal of neuroinflammation 2023 Nov 11;20(1):259

Tea Polyphenols Protects Tracheal Epithelial Tight Junctions in Lung during Actinobacillus pleuropneumoniae Infection via Suppressing TLR-4/MAPK/PKC-MLCK Signaling.

Li X, Liu Z, Gao T, Liu W, Yang K, Guo R, Li C, Tian Y, Wang N, Zhou D, Bei W, Yuan F
International journal of molecular sciences 2023 Jul 24;24(14)

Morphological and Functional Colonic Defects Caused by a Mutated Thyroid Hormone Receptor α.

Kim M, Kruhlak M, Hoffmann V, Zerfas P, Bishop K, Doolittle WKL, Edmondson EF, Zhu YJ, Cheng SY
Thyroid : official journal of the American Thyroid Association 2023 Feb;33(2):239-250

Vaccinia virus induces EMT-like transformation and RhoA-mediated mesenchymal migration.

Liu W, Lu JY, Wang YJ, Xu XX, Chen YC, Yu SX, Xiang XW, Chen XZ, Jiu Y, Gao H, Sheng M, Chen ZJ, Hu X, Li D, Maiuri P, Huang X, Ying T, Xu GL, Pang DW, Zhang ZL, Liu B, Liu YJ
Journal of medical virology 2023 Aug;95(8):e29041

Heat-Killed Lacticaseibacillus paracasei Repairs Lipopolysaccharide-Induced Intestinal Epithelial Barrier Damage via MLCK/MLC Pathway Activation.

Xie Z, Zhang G, Liu R, Wang Y, Tsapieva AN, Zhang L, Han J
Nutrients 2023 Apr 4;15(7)

Intestinal Flora-Derived Kynurenic Acid Protects Against Intestinal Damage Caused by Candida albicans Infection via Activation of Aryl Hydrocarbon Receptor.

Wang Z, Yin L, Qi Y, Zhang J, Zhu H, Tang J
Frontiers in microbiology 2022;13:934786

The m(6)A methyltransferase METTL3 promotes trophoblast cell invasion by regulating MYLK expression.

Zhao J, Ding H, Ding J, Shi X, He Y, Zhu H, Yuan H, Zhang T, Zhang J
Placenta 2022 Nov;129:1-6

Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.

He F, Ding H, Zhou Y, Wang Y, Xie J, Yang S, Zhu Y
Frontiers in medicine 2021;8:792740

Protective effects of curcumin against rat intestinal inflammation‑related motility disorders.

Yao Y, Luo R, Xiong S, Zhang C, Zhang Y
Molecular medicine reports 2021 May;23(5)

Lanthanum chloride causes blood-brain barrier disruption through intracellular calcium-mediated RhoA/Rho kinase signaling and myosin light chain kinase.

Wu J, Yang J, Yu M, Sun W, Han Y, Lu X, Jin C, Wu S, Cai Y
Metallomics : integrated biometal science 2020 Dec 23;12(12):2075-2083

Doxorubicin induces detrusor smooth muscle impairments through myosin dysregulation, leading to a risk of lower urinary tract dysfunction.

Iguchi N, Dönmez Mİ, Carrasco A Jr, Wilcox DT, Pineda RH, Malykhina AP, Cost NG
American journal of physiology. Renal physiology 2019 Jul 1;317(1):F197-F206

Aryl Hydrocarbon Receptor Activation Modulates Intestinal Epithelial Barrier Function by Maintaining Tight Junction Integrity.

Yu M, Wang Q, Ma Y, Li L, Yu K, Zhang Z, Chen G, Li X, Xiao W, Xu P, Yang H
International journal of biological sciences 2018;14(1):69-77

Dihydroartemisinin counteracts fibrotic portal hypertension via farnesoid X receptor-dependent inhibition of hepatic stellate cell contraction.

Xu W, Lu C, Zhang F, Shao J, Yao S, Zheng S
The FEBS journal 2017 Jan;284(1):114-133

Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment.

Sumagin R, Robin AZ, Nusrat A, Parkos CA
Mucosal immunology 2014 Jul;7(4):905-15

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse uterus tissue were subjected to SDS PAGE followed by western blot with 21642-1-AP(MYLK antibody) at dilution of 1:500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using MYLK antibody 21642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line