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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3182 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3182, RRID:AB_1577298
- Product name
- MYLK polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MYLK.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Analysis of the kinase-related protein gene found at human chromosome 3q21 in a multi-gene cluster: organization, expression, alternative splicing, and polymorphic marker.
A single human myosin light chain kinase gene (MLCK; MYLK).
Myosin light chain kinase in endothelium: molecular cloning and regulation.
Watterson DM, Schavocky JP, Guo L, Weiss C, Chlenski A, Shirinsky VP, Van Eldik LJ, Haiech J
Journal of cellular biochemistry 1999 Dec 1;75(3):481-91
Journal of cellular biochemistry 1999 Dec 1;75(3):481-91
A single human myosin light chain kinase gene (MLCK; MYLK).
Lazar V, Garcia JG
Genomics 1999 Apr 15;57(2):256-67
Genomics 1999 Apr 15;57(2):256-67
Myosin light chain kinase in endothelium: molecular cloning and regulation.
Garcia JG, Lazar V, Gilbert-McClain LI, Gallagher PJ, Verin AD
American journal of respiratory cell and molecular biology 1997 May;16(5):489-94
American journal of respiratory cell and molecular biology 1997 May;16(5):489-94
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MYLK polyclonal antibody (Cat # PAB3182) in mouse brain tissue lysate. MYLK (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with MYLK polyclonal antibody (Cat # PAB3182) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
